Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Elevated Plus Maze
Objective: Assess anxiety-like behavior in mice
This is a Elevated Plus Maze protocol using Mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2026 paper published in Chin Med.
Model and subjects
Mouse • C57BL/6J • Male • 8-week-old • 44
Study window
~1.1 hours hands-on
Core workflow
Pre-test habituation • Mouse placement • Recording and analysis
Primary readouts
- Time spent in open arms
- Distance the mice moved
- Number of times into the open arms
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Pre-test habituation
Transfer mice to testing room for acclimation
View evidence from paper
“On each test day, mice were transferred to the testing room 1 h in advance for acclimation”
Mouse placement
Place each mouse in the elevated plus maze apparatus
View evidence from paper
“Each mouse was placed in the apparatus for 5 min”
Recording and analysis
Record locomotion using video tracking software and analyze behavioral parameters
View evidence from paper
“Locomotion was recorded for 5 min using video tracking software (VisuTrack, shanghai, XR-VT)”