Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Sucrose Preference Test
Objective: Assess anhedonia-like behavior in mice by measuring preference for sucrose solution over water
This is a Sucrose Preference Test protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Chin Med.
Model and subjects
Mouse • C57BL/6J • Male • 8-week-old • Variable by experiment group
Study window
~26 hours hands-on
Core workflow
Pre-test deprivation • Setup bottles • Weigh containers
Primary readouts
- Sucrose preference percentage
- Sucrose consumption volume
- Water consumption volume
- Total fluid consumption
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Pre-test deprivation
Deprive mice of water and food
View evidence from paper
“After 24 h of water and food deprivation”
Setup bottles
Provide mice with two separate bottles of the same size, one containing 1% sucrose solution and one containing tap water
View evidence from paper
“two separate bottles of the same size: one containing a 1% sucrose solution and the other containing tap water”
Weigh containers
Weigh the containers before the test
View evidence from paper
“The containers were weighed”
Allow free consumption
Allow mice free access to both bottles
View evidence from paper
“fluid consumption after 2 h was calculated”
Calculate consumption
Calculate fluid consumption and determine sucrose preference using the formula: Sucrose preference = Sucrose consumption/(Sucrose consumption + water consumption) × 100
View evidence from paper
“Sucrose preference = Sucrose consumption/(Sucrose consumption + water consumption) × 100”