Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Sucrose Preference Test
Objective: Assess anhedonia-like behavior in mice by measuring preference for sucrose solution over water
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified
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Protocol Steps
Pre-test deprivation
Deprive mice of water and food
View evidence from paper
“After 24 h of water and food deprivation”
Setup bottles
Provide mice with two separate bottles of the same size, one containing 1% sucrose solution and one containing tap water
View evidence from paper
“two separate bottles of the same size: one containing a 1% sucrose solution and the other containing tap water”
Weigh containers
Weigh the containers before the test
View evidence from paper
“The containers were weighed”
Allow free consumption
Allow mice free access to both bottles
View evidence from paper
“fluid consumption after 2 h was calculated”
Calculate consumption
Calculate fluid consumption and determine sucrose preference using the formula: Sucrose preference = Sucrose consumption/(Sucrose consumption + water consumption) × 100
View evidence from paper
“Sucrose preference = Sucrose consumption/(Sucrose consumption + water consumption) × 100”