Source Paper
Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice
Han J, Xu X, Zhao Y, Xiao Y, Huang F et al.
Immunology • 2025
Rotarod Test
Objective: Assessment of motor coordination and balance in mice
This is a Rotarod Test protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items. Extracted from a 2025 paper published in Immunology.
Model and subjects
Mouse • B6SJL‐TgN(SOD1 G93A )1Gur/J transgenic mice • Not specified • 11, 14, 16, 18, and 20 weeks, as well as at the end stage • 60
Study window
Estimated timing pending
Core workflow
Initial setup • Gradual speed increase • Record fall time
Primary readouts
- Time until mouse falls from rotarod (average of three trials)
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Initial setup
Set the rotarod rotation to start at 4 rpm
View evidence from paper
“The rotation started at 4 rpm”
Gradual speed increase
Gradually increase the rotation speed to 40 rpm
View evidence from paper
“gradually increased to 40 rpm”
Record fall time
Record the time until the mouse falls from the rotating rod
View evidence from paper
“The time until the mouse fell was recorded”
Multiple trials
Perform three trials per mouse
View evidence from paper
“Each mouse underwent three trials”
Calculate average
Calculate the average time from the three trials
View evidence from paper
“the average time was calculated”