Source Paper
Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice
Han J, Xu X, Zhao Y, Xiao Y, Huang F et al.
Immunology • 2025
Rotarod Test
behavioralMouseB6SJL‐TgN(SOD1 G93A )1Gur/J transgenic mice
Objective: Assessment of motor coordination and balance in mice
Materials & Equipment Checklist
1 item1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Ugo Basile • Not specified
1 item available from ConductScience
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Protocol Steps
1
Initial setup
Set the rotarod rotation to start at 4 rpm
Not specified
View evidence from paper
“The rotation started at 4 rpm”
2
Gradual speed increase
Gradually increase the rotation speed to 40 rpm
Not specified
View evidence from paper
“gradually increased to 40 rpm”
3
Record fall time
Record the time until the mouse falls from the rotating rod
Until mouse falls
View evidence from paper
“The time until the mouse fell was recorded”
4
Multiple trials
Perform three trials per mouse
Not specified
View evidence from paper
“Each mouse underwent three trials”
5
Calculate average
Calculate the average time from the three trials
Not specified
View evidence from paper
“the average time was calculated”