Entorhinal Cortex Lesion
Objective: Examine the effects of entorhinal cortex lesioning on cell birth and neurogenesis in the rat dentate gyrus, and evaluate how afferent input and NMDA receptor activation regulate adult neurogenesis
This is a Entorhinal Cortex Lesion protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 3 materials. Extracted from a 1995 paper published in Journal of Neuroscience.
Model and subjects
rat • not specified • unknown • adult • not specified
Study window
Estimated timing pending
Core workflow
Entorhinal cortex lesioning • Assessment of cell birth and neurogenesis
Primary readouts
- Number of cells synthesizing DNA
- Number of cells in S phase identified with 3H-thymidine
- Birth of neurons
- Overall density of neurons in the granule cell layer
Key equipment and reagents
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Protocol Steps
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Entorhinal cortex lesioning
Surgical lesion of the entorhinal cortex, which is the main excitatory afferent population to the granule neurons of the dentate gyrus
Note: This lesion serves to remove excitatory input to the dentate gyrus
View evidence from paper
“Lesion of the entorhinal cortex, the main excitatory afferent population to the granule neurons, also increased the birth of cells in the dentate gyrus”
Assessment of cell birth and neurogenesis
Evaluate the effects of entorhinal cortex lesioning on the birth of cells in the dentate gyrus and overall neurogenesis
Note: Measurements include number of cells synthesizing DNA, cells in S phase, and overall density of neurons in granule cell layer
View evidence from paper
“increased the birth of cells in the dentate gyrus”