Source Paper
Pharmacological Evidence for a Motivational Role of κ-Opioid Systems in Ethanol Dependence
Brendan M Walker, George F Koob
Neuropsychopharmacology • 2007
Ethanol Vapor Exposure
Objective: To induce ethanol dependence in animals through intermittent ethanol vapor exposure over 4 weeks, with 14 hours of daily exposure
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Ethanol vapor exposure initiation
Begin intermittent ethanol vapor exposure protocol on animals designated for the dependent group
Note: Only half of the animals received this treatment; the other half served as controls
View evidence from paper
“Dependence was induced in half the animals by subjecting them to a 4-week intermittent vapor exposure period”
Daily vapor exposure
Expose animals to ethanol vapor for 14 hours per day throughout the 4-week period
Note: This is an intermittent exposure protocol, meaning exposure occurs daily but not continuously
View evidence from paper
“in which animals were exposed to ethanol vapor for 14 h per day”
Completion of dependence induction
After 4 weeks of intermittent vapor exposure, animals should have developed ethanol dependence
Note: Subsequent behavioral testing (self-administration) would follow this phase
View evidence from paper
“Subsequent to dependence induction, nalmefene, naltrexone, and nor-BNI were tested”