Source Paper
Pharmacological Evidence for a Motivational Role of κ-Opioid Systems in Ethanol Dependence
Brendan M Walker, George F Koob
Neuropsychopharmacology • 2007
Ethanol Vapor Exposure
Objective: To induce ethanol dependence in animals through intermittent ethanol vapor exposure over 4 weeks, with 14 hours of daily exposure
This is a Ethanol Vapor Exposure protocol using rat as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2007 paper published in Neuropsychopharmacology.
Model and subjects
rat • Wistar • male • Not specified • Not specified
Study window
~4 week study window | ~28 hours hands-on
Core workflow
Ethanol vapor exposure initiation • Daily vapor exposure • Completion of dependence induction
Primary readouts
- Ethanol dependence status (vapor-exposed vs control animals)
- Subsequent ethanol self-administration behavior (measured as lever presses)
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Ethanol vapor exposure initiation
Begin intermittent ethanol vapor exposure protocol on animals designated for the dependent group
Note: Only half of the animals received this treatment; the other half served as controls
View evidence from paper
“Dependence was induced in half the animals by subjecting them to a 4-week intermittent vapor exposure period”
Daily vapor exposure
Expose animals to ethanol vapor for 14 hours per day throughout the 4-week period
Note: This is an intermittent exposure protocol, meaning exposure occurs daily but not continuously
View evidence from paper
“in which animals were exposed to ethanol vapor for 14 h per day”
Completion of dependence induction
After 4 weeks of intermittent vapor exposure, animals should have developed ethanol dependence
Note: Subsequent behavioral testing (self-administration) would follow this phase
View evidence from paper
“Subsequent to dependence induction, nalmefene, naltrexone, and nor-BNI were tested”