Exercise Intervention Study
Objective: To identify pathways compromised in insulin resistance and test the effect of moderate exercise on whole-body and cellular insulin action in type 2 diabetic patients, obese nondiabetics, and lean controls
This is a Exercise Intervention Study protocol using human as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 1 materials. Extracted from a 2000 paper published in Journal of Clinical Investigation.
Model and subjects
human • N/A • unknown • Not specified • Not specified
Study window
~1 day study window | ~24 hours hands-on
Core workflow
Subject recruitment and stratification • Exercise intervention • Euglycemic clamp procedure
Primary readouts
- Phosphorylation of insulin receptor
- Phosphorylation of IRS-1
- IRS protein association with p85
- IRS protein association with PI 3-kinase
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Subject recruitment and stratification
Recruit and stratify subjects into three groups: type 2 diabetic patients, obese nondiabetics, and lean controls
Note: Three distinct subject populations were studied
View evidence from paper
“We conducted euglycemic clamps and muscle biopsies on type 2 diabetic patients, obese nondiabetics and lean controls”
Exercise intervention
Administer a single bout of moderate exercise to subjects
Note: Exercise was performed 24 hours before the euglycemic clamp measurement
View evidence from paper
“Exercise 24 hours before the euglycemic clamp increased phosphorylation of insulin receptor and IRS-1”
Euglycemic clamp procedure
Conduct euglycemic clamp to measure whole-body insulin action and glucose disposal 24 hours after exercise intervention
Note: Performed with and without prior exercise bout
View evidence from paper
“We conducted euglycemic clamps and muscle biopsies on type 2 diabetic patients, obese nondiabetics and lean controls, with and without a single bout of exercise”
Muscle biopsy collection
Obtain muscle tissue samples during or after euglycemic clamp for cellular analysis
Note: Biopsies were collected in conjunction with euglycemic clamp procedures
View evidence from paper
“We conducted euglycemic clamps and muscle biopsies on type 2 diabetic patients, obese nondiabetics and lean controls”
Measure phosphorylation of insulin receptor and IRS-1
Analyze muscle tissue samples for phosphorylation of insulin receptor and IRS-1 protein
Note: Measured in response to insulin stimulation
View evidence from paper
“Insulin stimulation of the phosphatidylinositol 3-kinase (PI 3-kinase) pathway, as measured by phosphorylation of the insulin receptor and IRS-1”
Measure IRS protein association with p85 and PI 3-kinase
Analyze muscle tissue for IRS protein association with p85 and PI 3-kinase regulatory subunit
Note: Association measured as indicator of PI 3-kinase pathway activation
View evidence from paper
“by IRS protein association with p85 and with PI 3-kinase”
Measure MAP kinase pathway activation
Assess insulin stimulation of the MAP kinase pathway in muscle tissue samples
Note: Compared between subject groups to determine differential pathway effects
View evidence from paper
“Insulin stimulation of the MAP kinase pathway was normal in obese and diabetic subjects”
Correlate glucose disposal with signaling markers
Analyze correlation between insulin-stimulated glucose disposal and association of p85 with IRS-1
Note: Identifies key steps in insulin resistance mechanism
View evidence from paper
“Insulin stimulation of glucose-disposal correlated with association of p85 with IRS-1”