Source Paper
Auditory Thalamus, Dorsal Hippocampus, Basolateral Amygdala, and Perirhinal Cortex Role in the Consolidation of Conditioned Freezing to Context and to Acoustic Conditioned Stimulus in the Rat
Benedetto Sacchetti, Carlo Ambrogi Lorenzini, Elisabetta Baldi, Giovanna Tassoni, Corrado Bucherelli
Journal of Neuroscience • 1999
Fear Conditioning Training
Objective: To assess the involvement of auditory thalamus, dorsal hippocampus, basolateral amygdala, and perirhinal cortex in the consolidation phase of conditioned freezing to both acoustic conditioned stimulus and context in rats
Gather these items before starting the experiment. Check off items as you prepare.
2 items available from ConductScience
Estimated: $5,900.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Fear conditioning training
Adult male Wistar rats undergo CS and context fear training to establish conditioned freezing responses
Note: Training must be completed before TTX injection phase
View evidence from paper
“adult male Wistar rats having undergone CS and context fear training”
Animal anesthesia
Anesthetize animals prior to stereotaxic injection
Note: Anesthesia type and dosage not specified in text
View evidence from paper
“Anesthetized animals were injected stereotaxically with TTX”
Stereotaxic TTX injection
Inject TTX stereotaxically into target brain structures (auditory thalamus, dorsal hippocampus, basolateral amygdala, or perirhinal cortex) at varying post-acquisition delays
Note: Injection volume and dose adjusted according to site dimensions. Injections performed at increasing post-acquisition delays to assess consolidation timing
View evidence from paper
“Anesthetized animals were injected stereotaxically with TTX (either 5 or 10 ng in 0.5 or 1.0 µl of saline, according to site dimensions) at increasing post-acquisition delays”
Retention testing
Measure context and CS freezing durations during retention testing
Note: Testing performed at both 48 and 72 hours post-injection to assess memory retention
View evidence from paper
“Context and CS freezing durations were measured during retention testing, always performed 48 and 72 hr after TTX administration”