Fear Conditioning with Lesions
Objective: Determine whether the lateral amygdaloid nucleus (AL) is the sensory interface of the amygdala in emotional conditioning by examining effects of AL lesions on classical conditioning of emotional responses to auditory stimuli
This is a Fear Conditioning with Lesions protocol using rat as the model organism. The procedure involves 3 procedural steps. Extracted from a 1990 paper published in Journal of Neuroscience.
Model and subjects
rat
Study window
Estimated timing pending
Core workflow
Lesion placement in lateral amygdaloid nucleus • Classical conditioning with auditory stimuli • Assessment of emotional conditioning effects
Primary readouts
- Emotional conditioning to auditory stimuli
- Effects of AL lesions on fear conditioning
- Responses following nonassociative training
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Lesion placement in lateral amygdaloid nucleus
Lesions were placed in the lateral amygdaloid nucleus (AL) of rats to examine effects on emotional conditioning
Note: Lesions of AL, but not lesions of the striatum above or the cortex adjacent to the AL, interfered with emotional conditioning. Lesions that only partially destroyed AL or lesions placed too ventrally that completely missed AL had no effect.
View evidence from paper
“Lesions were placed in AL of rats and the effects on emotional conditioning were examined. Lesions of AL, but not lesions of the striatum above or the cortex adjacent to the AL, interfered with emotional conditioning.”
Classical conditioning with auditory stimuli
Rats underwent classical conditioning of emotional responses to auditory stimuli following lesion placement
Note: The study examined whether the lateral amygdaloid nucleus is a major subcortical target of projections from the acoustic thalamus involved in emotional conditioning
View evidence from paper
“Previous work has implicated projections from the acoustic thalamus to the amygdala in the classical conditioning of emotional responses to auditory stimuli.”
Assessment of emotional conditioning effects
Examination of whether AL lesions interfered with emotional conditioning to auditory stimuli
Note: AL lesions did not affect the responses elicited following nonassociative (random) training, indicating specificity to associative conditioning
View evidence from paper
“AL lesions did not affect the responses elicited following nonassociative (random) training.”