Source Paper
No effect of partial reinforcement on fear extinction learning and memory in C57BL/6J mice
Su CJ, Fukunaga Y, Penna S, Cazares VA
Learn Mem • 2025
Fear Memory Retention Test
Objective: To assess the strength of recent and remote fear extinction memory following Pavlovian fear conditioning
This is a Fear Memory Retention Test protocol using Mice as the model organism. The procedure involves 5 procedural steps, 3 equipment items, 3 materials. Extracted from a 2025 paper published in Learn Mem.
Model and subjects
Mice • C67BL/6J • Male and female • Not specified • Not specified
Study window
~4.3 week study window
Core workflow
Fear Conditioning Training • Fear Extinction Training • Recent Memory Test
Primary readouts
- Percent time freezing during conditioned stimulus presentation
- Motion index based on pixel intensity variance across video frames
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Fear Conditioning Training
Conduct delayed fear conditioning with tone-shock pairings in context A (metal rod floor, vinegar scent, lights on)
View evidence from paper
“All experiments consisted of one or 2 days of delayed FC, during which 10 or 25 sec tones”
Fear Extinction Training
Administer 1-3 consecutive days of extinction training with 12 unreinforced tone exposures per day in context B
View evidence from paper
“Following FC, mice received between one and three consecutive days of FE training, each day of which consisted of 12 unreinforced exposures”
Recent Memory Test
Test fear memory retention by exposing mice to 4 conditioned stimuli at 2 days after extinction training
View evidence from paper
“To assess the strength of recent and remote FE memory, mice were exposed to four CS at 2 and 30 days”
Remote Memory Test
Test long-term fear memory retention by exposing mice to 4 conditioned stimuli at 30 days after extinction training
View evidence from paper
“mice were exposed to four CS at 2 and 30 days after extinction training”
Context Setup
Conduct memory tests in context B with semicircular white walls, white flooring, ethanol scent, and lights off
View evidence from paper
“FE and the 4 CS tone-tests were conducted in context B: a semicircular white acrylic wall”