Footfault Test
Objective: Assessment of sensorimotor functional recovery and motor coordination following traumatic brain injury
This is a Footfault Test protocol using rat as the model organism. The procedure involves 8 procedural steps, 2 materials. Extracted from a 2015 paper published in Journal of neurosurgery.
Model and subjects
rat • Wistar • 8
Study window
~5 week study window
Core workflow
Traumatic Brain Injury (TBI) Induction • Exosome or Vehicle Administration • Footfault Test
Primary readouts
- Sensorimotor functional recovery
- Motor coordination
- Cognitive function (Morris water maze)
- Neurological severity score
Key equipment and reagents
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Protocol Steps
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Traumatic Brain Injury (TBI) Induction
Wistar rats were subjected to TBI
Note: Specific TBI methodology not detailed in provided text
View evidence from paper
“Wistar rats were subjected to TBI”
Exosome or Vehicle Administration
Tail vein injection of either 100 µg protein of exosomes derived from MSCs or equal volume of vehicle phosphate-buffered saline
Note: Two treatment groups: exosome-treated (n=8) and vehicle control (n=8)
View evidence from paper
“tail vein injection of 100 µg protein of exosomes derived from MSCs or an equal volume of vehicle phosphate-buffered saline (n = 8/group) 24 hours later”
Footfault Test
Behavioral assessment of sensorimotor functional recovery and motor coordination
Note: Specific procedural details for footfault test not provided in text
View evidence from paper
“footfault tests were performed”
Modified Morris Water Maze
Cognitive and sensorimotor functional recovery evaluation
Note: Specific procedural details not provided in text
View evidence from paper
“the modified Morris water maze, neurological severity score and footfault tests were performed”
Neurological Severity Score Assessment
Evaluation of neurological function and recovery
Note: Specific procedural details not provided in text
View evidence from paper
“neurological severity score and footfault tests were performed”
Animal Sacrifice
Rats were sacrificed for tissue analysis
View evidence from paper
“Animals were sacrificed at 35 days after TBI”
Histopathological Analysis
Measurement of lesion volume
View evidence from paper
“Histopathological and immunohistochemical analyses were performed for measurements of lesion volume”
Immunohistochemical Analysis
Assessment of neurovascular remodeling (angiogenesis and neurogenesis) and neuroinflammation
View evidence from paper
“immunohistochemical analyses were performed for measurements of lesion volume, neurovascular remodeling (angiogenesis and neurogenesis), and neuroinflammation”