Source Paper
Temporally Graded Retrograde Amnesia of Contextual Fear after Hippocampal Damage in Rats: Within-Subjects Examination
Stephan G. Anagnostaras, Stephen Maren, Michael S. Fanselow
Journal of Neuroscience • 1999
Freezing Behavior Test
Objective: Measure freezing responses to assess contextual and tone fear memory retention after hippocampal lesions, examining retrograde amnesia in a within-subjects design
This is a Freezing Behavior Test protocol using rat as the model organism. The procedure involves 4 procedural steps. Extracted from a 1999 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~7.1 week study window
Core workflow
Remote Memory Fear Conditioning • Recent Memory Fear Conditioning • Hippocampal Lesion or Sham Surgery
Primary readouts
- Remote contextual fear freezing
- Recent contextual fear freezing
- Tone freezing responses
Key equipment and reagents
Verified items
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Protocol Steps
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Remote Memory Fear Conditioning
Rats received tone-shock pairings in one context to establish remote memory
Note: 10 tone-shock pairings administered in first context
View evidence from paper
“Rats were given 10 tone–shock pairings in one context (remote memory)”
Recent Memory Fear Conditioning
Rats received tone-shock pairings in a distinct context with a different tone to establish recent memory
Note: 10 tone-shock pairings in distinct context with different tone
View evidence from paper
“10 tone–shock pairings in a distinct context (with a different tone) 50 d later (recent memory)”
Hippocampal Lesion or Sham Surgery
Dorsal hippocampus electrolytic lesions or sham lesions performed
Note: DH or sham lesions administered; lesion timing is critical
View evidence from paper
“followed by DH or sham lesions 1 d later”
Freezing Behavior Assessment
Measure freezing responses to assess contextual and tone fear memory retention
Note: Freezing measured for both remote and recent contextual memory, and tone freezing
View evidence from paper
“DH-lesioned rats exhibited no deficit in remote contextual fear, but recent contextual fear memory was severely impaired. They also did not exhibit deficits in tone freezing”