Source Paper
Effects of Intrathecal Ketamine in the Neonatal Rat
Suellen M. Walker, B. David Westin, Ronald Deumens, Marjorie Grafe, Tony L. Yaksh
Anesthesiology • 2010
Gait Analysis
Objective: Assessment of gait parameters in rat pups at P35 following early postnatal intrathecal ketamine or saline administration
This is a Gait Analysis protocol using rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 7 materials. Extracted from a 2010 paper published in Anesthesiology.
Model and subjects
rat • not specified • unknown • P35 (postnatal day 35) at time of gait analysis; treated at P3, P7, or P21 • not specified
Study window
Estimated timing pending
Core workflow
Anesthetize rat pups • Administer intrathecal ketamine or saline • Evaluate mechanical withdrawal threshold
Primary readouts
- Mechanical withdrawal threshold
- Antinociceptive effects
- Anti-hyperalgesic effects
- Apoptosis markers (Fluoro-Jade C and activated caspase-3)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Anesthetize rat pups
Anesthetize rat pups prior to intrathecal injection
Note: Anesthetic type and dose not specified
View evidence from paper
“In anesthetized rat pups, intrathecal ketamine was administered by lumbar percutaneous injection”
Administer intrathecal ketamine or saline
Perform lumbar percutaneous injection of ketamine or saline at maximal doses
Note: Administration timing: P3 or P21; maximal doses used
View evidence from paper
“intrathecal ketamine was administered by lumbar percutaneous injection. Following maximal doses of ketamine or saline at P3 or P21”
Evaluate mechanical withdrawal threshold
Assess dose-dependent antinociceptive and carrageenan-induced anti-hyperalgesic effects
Note: Testing performed at P3 and P21
View evidence from paper
“Changes in mechanical withdrawal threshold evaluated dose-dependent antinociceptive and carrageenan-induced anti-hyperalgesic effects in postnatal day (P)3 and 21 rat pups”
Examine spinal cord tissue
Collect and examine spinal cords for apoptosis, histopathological changes, and glial responses
Note: Performed at P3, P7, or P21 following ketamine administration
View evidence from paper
“Following intrathecal ketamine at P3, 7 or 21, spinal cords were examined for apoptosis (Fluoro-Jade C and activated caspase-3), histopathological change, and glial responses”
Perform gait analysis
Evaluate gait parameters in rat pups at P35
Note: Performed at P35, following treatment at P3 or P21
View evidence from paper
“Following maximal doses of ketamine or saline at P3 or P21, sensory thresholds and gait analysis were evaluated at P35”