Source Paper
Facilitation of Conditioned Fear Extinction by Systemic Administration or Intra-Amygdala Infusions of d-Cycloserine as Assessed with Fear-Potentiated Startle in Rats
David L. Walker, Kerry J. Ressler, Kwok-Tung Lu, Michael Davis
Journal of Neuroscience • 2002
Glycine Site Antagonist (HA-966) Treatment
Objective: To evaluate whether HA-966, a glycine-recognition site antagonist, blocks the facilitating effects of D-cycloserine on conditioned fear extinction as measured by fear-potentiated startle in rats
This is a Glycine Site Antagonist (HA-966) Treatment protocol using rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 3 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
rat • not specified • not specified • not specified • not specified • not specified
Study window
Estimated timing pending
Core workflow
Fear Conditioning • Baseline Fear-Potentiated Startle Measurement • HA-966 Administration
Primary readouts
- Fear-potentiated startle response (increased startle in presence vs absence of light)
- Extinction learning as measured by reduction in fear-potentiated startle after non-reinforced light presentations
- Dose-dependent effects of DCS on extinction
- Blocking effects of HA-966 on DCS-facilitated extinction
Key equipment and reagents
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Protocol Steps
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Fear Conditioning
Rats received fear conditioning with paired presentations of light and footshock
Note: Light stimulus duration: 3.7 seconds; Footshock intensity: 0.4 mA
View evidence from paper
“Rats received 10 pairings of a 3.7 sec light and a 0.4 mA footshock (fear conditioning)”
Baseline Fear-Potentiated Startle Measurement
Fear-potentiated startle was measured before extinction training to establish baseline response
Note: Measured as increased startle in presence vs absence of light
View evidence from paper
“Fear-potentiated startle (increased startle in the presence vs the absence of the light) was subsequently measured before and after”
HA-966 Administration
HA-966, a glycine-recognition site antagonist, was administered to block D-cycloserine effects
Note: Specific dose and route not detailed in provided text
View evidence from paper
“These effects were blocked by HA-966, an antagonist at the glycine-recognition site (experiment 4)”
Extinction Training
Rats received non-reinforced light presentations without shock to test extinction learning
Note: 30 presentations produced modest extinction; 60 or 90 presentations produced nearly complete extinction
View evidence from paper
“30, 60, or 90 presentations of the light without shock (extinction training). Thirty non-reinforced light presentations produced modest extinction, and 60 or 90 presentations produced nearly complete extinction”
Post-Extinction Fear-Potentiated Startle Measurement
Fear-potentiated startle was measured after extinction training to assess extinction learning
Note: Compared to baseline measurements
View evidence from paper
“Fear-potentiated startle (increased startle in the presence vs the absence of the light) was subsequently measured before and after”