Grid-walk Analysis
Objective: Assessment of functional recovery and motor performance in transplanted rats using grid-walk testing to evaluate the effects of neural precursor cell transplantation on spinal cord injury recovery
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
not specified • not mentioned • not mentioned • not mentioned
Materials4
Software1
not specified • not mentioned
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Protocol Steps
Spinal cord injury induction
Adult rats received spinal cord injury
Note: Injury was performed at baseline before transplantation
View evidence from paper
“transplanted adult brain-derived neural precursor cells (NPCs) isolated from yellow fluorescent protein-expressing transgenic mice into the injured spinal cord of adult rats”
Neural precursor cell transplantation
Adult brain-derived NPCs were transplanted into the injured spinal cord at two different time points
Note: Transplantation performed at subacute (2 weeks) and chronic (8 weeks) phases post-injury
View evidence from paper
“transplanted adult brain-derived neural precursor cells (NPCs) isolated from yellow fluorescent protein-expressing transgenic mice into the injured spinal cord of adult rats at 2 and 8 weeks after injury, which represents the subacute and chronic phases of SCI”
Pharmacological treatment
Administration of growth factors, minocycline, and cyclosporine A to enhance NPC survival
Note: Treatment was combined to enhance survival of transplanted cells
View evidence from paper
“A combination of growth factors, the anti-inflammatory drug minocycline, and cyclosporine A immunosuppression was used to enhance the survival of transplanted adult NPCs”
Grid-walk testing
Assessment of motor performance and functional recovery using grid-walk analysis
Note: Testing was performed to evaluate functional recovery in transplanted rats
View evidence from paper
“injured rats receiving NPC transplants had improved functional recovery as assessed by the Basso, Beattie, and Bresnahan Locomotor Rating Scale and grid-walk and footprint analyses”
Footprint analysis
Assessment of motor performance using footprint analysis in conjunction with grid-walk testing
Note: Complementary assessment method used alongside grid-walk and locomotor rating scale
View evidence from paper
“injured rats receiving NPC transplants had improved functional recovery as assessed by the Basso, Beattie, and Bresnahan Locomotor Rating Scale and grid-walk and footprint analyses”