Grip Strength Test
Objective: Measurement of grip strength to evaluate motor function recovery after permanent middle cerebral artery occlusion (stroke) in rats
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Protocol Steps
Surgical Procedure - Permanent Middle Cerebral Artery Occlusion
Male Sprague-Dawley rats (12 months old) underwent permanent middle cerebral artery occlusion or sham operations
Note: This is the stroke induction procedure; sham-operated animals served as controls
View evidence from paper
“We tested male Sprague-Dawley rats (12 months old) with permanent middle cerebral artery occlusion or sham operations”
Dose-Response Study - Initial Progesterone Administration
Animals received intraperitoneal injections of progesterone at 1 hour post-occlusion
Note: Three dose levels tested: 8, 16, or 32 mg/kg
View evidence from paper
“animals received intraperitoneal injections of progesterone (8, 16 or 32 mg/kg) at 1 h post-occlusion”
Dose-Response Study - Subcutaneous Injections
Animals received subcutaneous injections of progesterone at 6 hours post-occlusion and then once every 24 hours for 7 days
Note: Same dose levels as intraperitoneal injections
View evidence from paper
“subcutaneous injections at 6 h and then once every 24 h for 7 days”
Time-Window Study - Progesterone Administration
Optimal dose of progesterone was administered starting at 3, 6, or 24 hours post-stroke
Note: The optimal dose (8 mg/kg) was determined from the dose-response study
View evidence from paper
“For the time-window study, the optimal dose of progesterone was given starting at 3, 6 or 24 h post-stroke”
Behavioral Testing - Grip Strength Assessment
Grip strength was measured at repeated intervals to evaluate motor function recovery
Note: Part of comprehensive behavioral assessment including locomotor activity, sensory neglect, gait impairment, motor coordination, and spatial navigation
View evidence from paper
“Behavioural recovery was evaluated at repeated intervals. improved functional outcomes up to 3 weeks after stroke on locomotor activity, grip strength, sensory neglect, gait impairment, motor coordination and spatial navigation tests”
Brain Extraction and Infarct Volume Analysis
Rats were killed at 22 days post-stroke and brains extracted for evaluation of infarct volume
Note: Terminal procedure for histological analysis
View evidence from paper
“Rats were killed at 22 days post-stroke and brains extracted for evaluation of infarct volume”