Hippocampal X-irradiation and Neurogenesis Ablation
Objective: To determine whether X-irradiation of the hippocampus blocks both the neurogenic and behavioral effects of chronic HU210 treatment, establishing that hippocampal neurogenesis is necessary for the anxiolytic and antidepressant-like effects of cannabinoids
This is a Hippocampal X-irradiation and Neurogenesis Ablation protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2005 paper published in Journal of Clinical Investigation.
Model and subjects
rat • Not specified • unknown • adult • Not specified
Study window
Estimated timing pending
Core workflow
Chronic HU210 treatment • Hippocampal X-irradiation • Behavioral assessment
Primary readouts
- Neurogenesis in hippocampal dentate gyrus (with and without X-irradiation)
- Anxiolytic-like behavioral effects (with and without X-irradiation)
- Antidepressant-like behavioral effects (with and without X-irradiation)
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Chronic HU210 treatment
Administer chronic HU210 treatment to adult rats
Note: Chronic, but not acute, HU210 treatment was used in this study
View evidence from paper
“Chronic, but not acute, HU210 treatment promoted neurogenesis in the hippocampal dentate gyrus of adult rats”
Hippocampal X-irradiation
Deliver X-irradiation to the hippocampus to ablate neurogenesis
Note: X-irradiation was used to block neurogenesis in the hippocampus prior to or concurrent with HU210 treatment
View evidence from paper
“X-irradiation of the hippocampus blocked both the neurogenic and behavioral effects of chronic HU210 treatment”
Behavioral assessment
Assess anxiolytic and antidepressant-like behavioral effects following HU210 treatment with and without X-irradiation
Note: Behavioral testing was conducted to measure anxiolytic and antidepressant-like effects
View evidence from paper
“exerted anxiolytic- and antidepressant-like effects. X-irradiation of the hippocampus blocked both the neurogenic and behavioral effects”
Neurogenesis assessment
Measure neurogenesis in the hippocampal dentate gyrus following HU210 treatment with and without X-irradiation
Note: Neurogenesis was assessed in the hippocampal dentate gyrus to determine if X-irradiation blocked the neurogenic effects
View evidence from paper
“X-irradiation of the hippocampus blocked both the neurogenic and behavioral effects of chronic HU210 treatment”