IL-6 Infusion In Vivo
Objective: To explore the hypothesis that elevated levels of IL-6 mediate adverse myocardial remodeling by infusing IL-6 in vivo and assessing left ventricular function, myocardial collagen content, and cardiomyocyte dimensions
This is a IL-6 Infusion In Vivo protocol using rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 2 materials. Extracted from a 2010 paper published in Hypertension.
Model and subjects
rat • Sprague Dawley • male • adult
Study window
~1 week study window
Core workflow
IL-6 infusion via osmotic minipump • Left ventricular function evaluation • Myocardial collagen assessment
Primary readouts
- Concentric left ventricular hypertrophy
- Increased ventricular stiffness
- Collagen volume fraction (measured as percentage)
- Cardiomyocyte width
Key equipment and reagents
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Protocol Steps
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IL-6 infusion via osmotic minipump
Adult male Sprague Dawley rats received continuous intraperitoneal infusion of IL-6 at a dose of 2.5 µg·kg⁻¹·hr⁻¹ using an osmotic minipump
Note: Aged-matched control rats received vehicle infusion via the same method
View evidence from paper
“adult male Sprague Dawley rats were infused with IL-6 (2.5 µg·kg⁻¹·hr⁻¹, IP) for 7 days via osmotic minipump and compared to vehicle infused aged-matched controls”
Left ventricular function evaluation
After the 7-day infusion period, left ventricular function was assessed using a blood-perfused isolated heart preparation
View evidence from paper
“Left ventricular function was evaluated using a blood-perfused isolated heart preparation”
Myocardial collagen assessment
Myocardial interstitial collagen volume fraction was measured in tissue samples
View evidence from paper
“myocardial interstitial collagen volume fraction and isolated cardiomyocyte size were also assessed”
Cardiomyocyte size measurement
Isolated cardiomyocyte size was assessed, including width and length measurements
View evidence from paper
“isolated cardiomyocyte size were also assessed”
Isolated cardiac fibroblast experiments
Isolated adult cardiac fibroblast experiments were performed to determine the importance of the soluble IL-6 receptor in mediating cardiac fibrosis
View evidence from paper
“Isolated adult cardiac fibroblast experiments were performed to determine the importance of the soluble IL-6 receptor in mediating cardiac fibrosis”