In Vivo Antibody Infusion in Rats
Objective: Demonstrate that patients' NMDAR antibodies cause selective and reversible decrease in NMDAR surface density and synaptic localization in the hippocampus of female Lewis rats, correlating with antibody titers
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
not specified • not applicable • not mentioned • not mentioned
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Protocol Steps
Antibody infusion into female Lewis rats
Infuse patients' NMDAR antibodies into female Lewis rats
Note: Infusion method, volume, and injection site not detailed in provided text
View evidence from paper
“NMDAR density was dramatically reduced in the hippocampus of female Lewis rats infused with patients' antibodies”
Hippocampal tissue analysis
Measure NMDAR density in the hippocampus following antibody infusion
Note: Specific analytical methods for measuring NMDAR density not detailed in provided text
View evidence from paper
“NMDAR density was dramatically reduced in the hippocampus of female Lewis rats infused with patients' antibodies, similar to the decrease observed in the hippocampus of autopsied patients”