In Vivo Antibody Infusion in Rats
Objective: Demonstrate that patients' NMDAR antibodies cause selective and reversible decrease in NMDAR surface density and synaptic localization in the hippocampus of female Lewis rats, correlating with antibody titers
This is a In Vivo Antibody Infusion in Rats protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 materials. Extracted from a 2010 paper published in Journal of Neuroscience.
Model and subjects
rat • Lewis • female • not specified • not specified
Study window
Estimated timing pending
Core workflow
Antibody infusion into female Lewis rats • Hippocampal tissue analysis
Primary readouts
- NMDAR surface density reduction in hippocampus
- NMDAR synaptic localization
- Correlation with patients' antibody titers
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Antibody infusion into female Lewis rats
Infuse patients' NMDAR antibodies into female Lewis rats
Note: Infusion method, volume, and injection site not detailed in provided text
View evidence from paper
“NMDAR density was dramatically reduced in the hippocampus of female Lewis rats infused with patients' antibodies”
Hippocampal tissue analysis
Measure NMDAR density in the hippocampus following antibody infusion
Note: Specific analytical methods for measuring NMDAR density not detailed in provided text
View evidence from paper
“NMDAR density was dramatically reduced in the hippocampus of female Lewis rats infused with patients' antibodies, similar to the decrease observed in the hippocampus of autopsied patients”