Source Paper
Mechanism of Toxicity in Rotenone Models of Parkinson's Disease
Todd B. Sherer, Ranjita Betarbet, Claudia M. Testa, Byoung Boo Seo, Jason R. Richardson et al.
Journal of Neuroscience • 2003
In Vivo Rotenone Treatment in Rats
Objective: Examine mechanisms of rotenone toxicity and demonstrate involvement of oxidative damage in rotenone-induced dopaminergic neuronal death using three model systems of increasing complexity
This is a In Vivo Rotenone Treatment in Rats protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 2 materials. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Chronic rotenone exposure in rats • Brain tissue collection and analysis
Primary readouts
- Oxidative damage in brain tissue
- Dopaminergic neuronal loss
- Selective nigrostriatal dopaminergic degeneration
- α-synuclein-positive cytoplasmic inclusions
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Chronic rotenone exposure in rats
Rats were exposed to rotenone to induce Parkinson's disease-like features including selective nigrostriatal dopaminergic degeneration and α-synuclein-positive cytoplasmic inclusions
Note: This is the primary in vivo model system. Specific dosage, route of administration, and treatment duration not detailed in provided text
View evidence from paper
“Exposure of rats to the pesticide and complex I inhibitor rotenone reproduces features of Parkinson's disease, including selective nigrostriatal dopaminergic degeneration and α-synuclein-positive cytoplasmic inclusions”
Brain tissue collection and analysis
Brains from rotenone-treated animals were examined for oxidative damage, particularly in midbrain and olfactory bulb regions
Note: Analysis focused on dopaminergic regions affected by Parkinson's disease
View evidence from paper
“brains from rotenone-treated animals demonstrated oxidative damage, most notably in midbrain and olfactory bulb, dopaminergic regions affected by Parkinson's disease”