Source Paper
Mechanism of Toxicity in Rotenone Models of Parkinson's Disease
Todd B. Sherer, Ranjita Betarbet, Claudia M. Testa, Byoung Boo Seo, Jason R. Richardson et al.
Journal of Neuroscience • 2003
In Vivo Rotenone Treatment in Rats
Objective: Examine mechanisms of rotenone toxicity and demonstrate involvement of oxidative damage in rotenone-induced dopaminergic neuronal death using three model systems of increasing complexity
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Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Chronic rotenone exposure in rats
Rats were exposed to rotenone to induce Parkinson's disease-like features including selective nigrostriatal dopaminergic degeneration and α-synuclein-positive cytoplasmic inclusions
Note: This is the primary in vivo model system. Specific dosage, route of administration, and treatment duration not detailed in provided text
View evidence from paper
“Exposure of rats to the pesticide and complex I inhibitor rotenone reproduces features of Parkinson's disease, including selective nigrostriatal dopaminergic degeneration and α-synuclein-positive cytoplasmic inclusions”
Brain tissue collection and analysis
Brains from rotenone-treated animals were examined for oxidative damage, particularly in midbrain and olfactory bulb regions
Note: Analysis focused on dopaminergic regions affected by Parkinson's disease
View evidence from paper
“brains from rotenone-treated animals demonstrated oxidative damage, most notably in midbrain and olfactory bulb, dopaminergic regions affected by Parkinson's disease”