Intra-VTA Orexin Receptor Antagonist Injection
Objective: To assess the effects of selective orexin receptor antagonist SB334867A injected directly into the ventral tegmental area (VTA) on morphine-induced place preference in rats
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Equipment2
Materials2
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Protocol Steps
Prepare orexin receptor antagonist solution
Prepare SB334867A solution for microinjection into the VTA
Note: SB334867A is a selective orexin receptor antagonist with chemical structure 1-(2-methylbenzoxazol-6-yl)-3-[1.5]naphthyridin-4-yl urea
View evidence from paper
“An intra-VTA injection of a selective orexin receptor antagonist SB334867A [1-(2-methylbenzoxazol-6-yl)-3-[1.5]naphthyridin-4-yl urea]”
Perform intra-VTA microinjection
Inject SB334867A directly into the ventral tegmental area of rats using microinjection technique
Note: Injection targets the ventral tegmental area where orexin receptors are coexpressed with tyrosine hydroxylase
View evidence from paper
“An intra-VTA injection of a selective orexin receptor antagonist SB334867A significantly suppressed the morphine-induced place preference in rats”
Administer morphine
Administer morphine subcutaneously to induce place preference and hyperlocomotion
Conditioned Place Preference - Mouse (Custom Order)
Matches: Place preference apparatus/chamber
Note: Morphine is a μ-opioid receptor agonist
View evidence from paper
“The subcutaneous morphine-induced place preference and hyperlocomotion observed in wild-type mice”
Measure place preference behavior
Assess morphine-induced place preference in the behavioral apparatus following SB334867A injection and morphine administration
Conditioned Place Preference - Mouse (Custom Order)
Matches: Place preference apparatus/chamber
Note: Place preference is measured as a behavioral outcome of the morphine-orexin system interaction
View evidence from paper
“An intra-VTA injection of a selective orexin receptor antagonist SB334867A significantly suppressed the morphine-induced place preference in rats”