Source Paper
Endocannabinoid levels in rat limbic forebrain and hypothalamus in relation to fasting, feeding and satiation: stimulation of eating by 2‐arachidonoyl glycerol
Tim C Kirkham, Claire M Williams, Filomena Fezza, Vincenzo Di Marzo
British Journal of Pharmacology • 2002
Intracranial Injection and Feeding Behavior
Objective: To examine the behavioral effects of 2-arachidonoyl glycerol (2-AG) when injected into the nucleus accumbens shell and measure its stimulation of feeding behavior, with assessment of CB1 receptor involvement
This is a Intracranial Injection and Feeding Behavior protocol using rat as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 2 materials. Extracted from a 2002 paper published in British Journal of Pharmacology.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Intracranial injection of 2-AG • Measure feeding behavior • CB1 receptor antagonist administration
Primary readouts
- Feeding behavior stimulation following 2-AG injection
- Dose-dependent effects of 2-AG on feeding
- Attenuation of feeding stimulation by CB1 receptor antagonist SR141716
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Intracranial injection of 2-AG
Inject 2-arachidonoyl glycerol into the nucleus accumbens shell of rats at varying doses to test dose-dependent effects
Note: Injection targets the nucleus accumbens shell, a limbic forebrain area linked to eating motivation
View evidence from paper
“we examined the behavioural effects of 2-AG when injected into the nucleus accumbens shell”
Measure feeding behavior
Observe and record feeding behavior following 2-AG injection to assess potency and dose-dependent stimulation of eating
Note: 2-AG potently and dose-dependently stimulates feeding
View evidence from paper
“2-AG potently, and dose-dependently, stimulated feeding”
CB1 receptor antagonist administration
Administer SR141716 (CB1 receptor antagonist) to determine if the feeding stimulation effect of 2-AG is mediated through CB1 receptors
Note: SR141716 attenuates the feeding stimulation effect, confirming CB1 receptor involvement
View evidence from paper
“This effect was attenuated by the CB1 receptor antagonist SR141716”