Source Paper
Endocannabinoid levels in rat limbic forebrain and hypothalamus in relation to fasting, feeding and satiation: stimulation of eating by 2‐arachidonoyl glycerol
Tim C Kirkham, Claire M Williams, Filomena Fezza, Vincenzo Di Marzo
British Journal of Pharmacology • 2002
Intracranial Injection and Feeding Behavior
Objective: To examine the behavioral effects of 2-arachidonoyl glycerol (2-AG) when injected into the nucleus accumbens shell and measure its stimulation of feeding behavior, with assessment of CB1 receptor involvement
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Intracranial injection of 2-AG
Inject 2-arachidonoyl glycerol into the nucleus accumbens shell of rats at varying doses to test dose-dependent effects
Note: Injection targets the nucleus accumbens shell, a limbic forebrain area linked to eating motivation
View evidence from paper
“we examined the behavioural effects of 2-AG when injected into the nucleus accumbens shell”
Measure feeding behavior
Observe and record feeding behavior following 2-AG injection to assess potency and dose-dependent stimulation of eating
Note: 2-AG potently and dose-dependently stimulates feeding
View evidence from paper
“2-AG potently, and dose-dependently, stimulated feeding”
CB1 receptor antagonist administration
Administer SR141716 (CB1 receptor antagonist) to determine if the feeding stimulation effect of 2-AG is mediated through CB1 receptors
Note: SR141716 attenuates the feeding stimulation effect, confirming CB1 receptor involvement
View evidence from paper
“This effect was attenuated by the CB1 receptor antagonist SR141716”