Intracranial Injection - Medial Prefrontal Cortex
Objective: To assess regional effects of GLYX-13 on antidepressant-like behavior when injected directly into the medial prefrontal cortex
This is a Intracranial Injection - Medial Prefrontal Cortex protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 2 materials. Extracted from a 2012 paper published in Neuropsychopharmacology.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Intracranial injection of GLYX-13 into medial prefrontal cortex • Behavioral testing following injection
Primary readouts
- Antidepressant-like behavior in Porsolt test
- Antidepressant-like behavior in novelty induced hypophagia test
- Antidepressant-like behavior in learned helplessness test
- Absence of substance abuse-related side effects
Key equipment and reagents
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Protocol Steps
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Intracranial injection of GLYX-13 into medial prefrontal cortex
Direct injection of GLYX-13 into the medial prefrontal cortex (MPFC) of rats
Note: This injection method differs from systemic administration and produces antidepressant-like effects
View evidence from paper
“GLYX-13, unlike ketamine, produced its antidepressant-like effect when injected directly into the medial prefrontal cortex”
Behavioral testing following injection
Assessment of antidepressant-like effects using behavioral tests
Note: Tests include Porsolt, novelty induced hypophagia, and learned helplessness tests
View evidence from paper
“produces an antidepressant-like effect in the Porsolt, novelty induced hypophagia, and learned helplessness tests in rats”