Brain-Derived Neurotrophic Factor Produces Antidepressant Effects in Behavioral Models of Depression

Yukihiko Shirayama, Andrew C.-H. Chen, Shin Nakagawa, David S. Russell, Ronald S. Duman

Journal of Neuroscience • 2002

Locomotor Activity Assay

behavioralrat

Objective: A control behavioral test to determine whether BDNF and NT-3 infusions influence general motor activity

Materials & Equipment Checklist

3 items

Gather these items before starting the experiment. Check off items as you prepare.

BDNF (Brain-Derived Neurotrophic Factor)

NT-3 (Neurotrophin-3)

Nerve Growth Factor (NGF)

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Administer bilateral infusions of BDNF, NT-3, or control compounds into the dentate gyrus of the hippocampus

Single infusion

Note: A single bilateral infusion was performed

View evidence from paper

“A single bilateral infusion of BDNF into the dentate gyrus of hippocampus produced an antidepressant effect”

Measure general motor activity following infusion to determine if BDNF and NT-3 influence locomotor behavior

Assessed at 3 days and up to 10 days post-infusion

Note: Locomotor activity was measured as a control behavioral test

View evidence from paper

“Infusions of BDNF and NT-3 did not influence locomotor activity or passive avoidance”