Source Paper
Brain-Derived Neurotrophic Factor Produces Antidepressant Effects in Behavioral Models of Depression
Yukihiko Shirayama, Andrew C.-H. Chen, Shin Nakagawa, David S. Russell, Ronald S. Duman
Journal of Neuroscience • 2002
Locomotor Activity Assay
Objective: A control behavioral test to determine whether BDNF and NT-3 infusions influence general motor activity
This is a Locomotor Activity Assay protocol using rat as the model organism. The procedure involves 2 procedural steps, 3 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
rat
Study window
~1.4 week study window
Core workflow
Bilateral infusion of test compounds • Locomotor activity assessment
Primary readouts
- Locomotor activity levels
- General motor activity
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Bilateral infusion of test compounds
Administer bilateral infusions of BDNF, NT-3, or control compounds into the dentate gyrus of the hippocampus
Note: A single bilateral infusion was performed
View evidence from paper
“A single bilateral infusion of BDNF into the dentate gyrus of hippocampus produced an antidepressant effect”
Locomotor activity assessment
Measure general motor activity following infusion to determine if BDNF and NT-3 influence locomotor behavior
Note: Locomotor activity was measured as a control behavioral test
View evidence from paper
“Infusions of BDNF and NT-3 did not influence locomotor activity or passive avoidance”