Source Paper
Rho Kinase Inhibition Enhances Axonal Regeneration in the Injured CNS
Alyson E. Fournier, Bayan T. Takizawa, Stephen M. Strittmatter
Journal of Neuroscience • 2003
Locomotor Recovery Assessment
Objective: Measurement of locomotor function recovery following corticospinal tract lesions in adult rats treated with ROCK inhibitor Y-27632
This is a Locomotor Recovery Assessment protocol using rat as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • adult • Not specified
Study window
Estimated timing pending
Core workflow
Corticospinal tract lesion creation • Y-27632 treatment administration • Locomotor recovery assessment
Primary readouts
- Locomotor recovery following CST lesions
- CST fiber sprouting
- Neurite outgrowth on myelin-associated inhibitors
Key equipment and reagents
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Protocol Steps
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Corticospinal tract lesion creation
Create CST lesions in adult rats
Note: Lesion method not detailed in provided text
View evidence from paper
“Y-27632 enhances sprouting of CST fibers in vivo and accelerates locomotor recovery after CST lesions in adult rats”
Y-27632 treatment administration
Administer ROCK inhibitor Y-27632 to lesioned rats
Note: Specific dosage, route, and timing of administration not provided in text
View evidence from paper
“Inhibition of p160ROCK with Y-27632 also promotes neurite outgrowth on myelin-associated inhibitors in vitro”
Locomotor recovery assessment
Measure locomotor function recovery in treated and control rats
Note: Specific assessment methods and timeline not detailed in provided text
View evidence from paper
“Y-27632 enhances sprouting of CST fibers in vivo and accelerates locomotor recovery after CST lesions in adult rats”