Source Paper
Early Exposure to Common Anesthetic Agents Causes Widespread Neurodegeneration in the Developing Rat Brain and Persistent Learning Deficits
Vesna Jevtovic-Todorovic, Richard E. Hartman, Yukitoshi Izumi, Nicholas D. Benshoff, Krikor Dikranian et al.
Journal of Neuroscience • 2003
Memory and Learning Impairment Testing
Objective: Assessment of persistent memory and learning impairments following early exposure to anesthetic agents (midazolam, nitrous oxide, and isoflurane) in developing rats
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Materials3
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Protocol Steps
Anesthetic administration
Administer combination of midazolam, nitrous oxide, and isoflurane to 7-day-old infant rats in doses sufficient to maintain a surgical plane of anesthesia
Note: Doses were sufficient to maintain surgical plane of anesthesia
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“administered to 7-d-old infant rats a combination of drugs commonly used in pediatric anesthesia (midazolam, nitrous oxide, and isoflurane) in doses sufficient to maintain a surgical plane of anesthesia for 6 hr”
Memory and learning assessment
Assess persistent memory and learning impairments following anesthetic exposure
Note: Testing conducted to evaluate persistent deficits
View evidence from paper
“persistent memory/learning impairments”
Hippocampal synaptic function evaluation
Evaluate deficits in hippocampal synaptic function
Note: Assessment of synaptic dysfunction following anesthetic exposure
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“deficits in hippocampal synaptic function, and persistent memory/learning impairments”