Mono-iodoacetate (MIA) Intra-articular Injection for OA Induction
Objective: To induce osteoarthritis and joint pain in rats through intra-articular injection of mono-iodoacetate (MIA) into the knee joint, creating an experimental OA model for studying joint pain mechanisms
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Protocol Steps
Animal acclimation
Rats were acclimated to the facility following arrival
Note: This step precedes anesthesia and injection
View evidence from paper
“After acclimation to the facility, rats were anesthetized via intraperitoneal injection of sodium pentobarbital”
Anesthesia induction
Rats were anesthetized via intraperitoneal injection of sodium pentobarbital prior to knee injection
Note: Anesthesia must be induced before the intra-articular injection procedure
View evidence from paper
“rats were anesthetized via intraperitoneal injection of sodium pentobarbital”
MIA preparation
Mono-iodoacetate (3 mg) was dissolved in 50 μl saline solution
Note: The MIA solution must be prepared fresh for injection
View evidence from paper
“3 mg of mono-iodoacetate (MIA) dissolved in 50 μl saline were injected into the left knee”
Intra-articular injection
The prepared MIA solution (3 mg in 50 μl saline) was injected into the left knee joint using a 27-gauge needle
Note: Injection is performed into the left knee joint specifically; the right knee serves as control
View evidence from paper
“3 mg of mono-iodoacetate (MIA) dissolved in 50 μl saline were injected into the left knee using a 27-gauge needle”