Source Paper
Nogo Receptor Antagonism Promotes Stroke Recovery by Enhancing Axonal Plasticity
Jung-Kil Lee, Ji-Eun Kim, Michael Sivula, Stephen M. Strittmatter
Journal of Neuroscience • 2004
Middle Cerebral Artery Occlusion with NgR Fragment Treatment
Objective: Examine whether blockade of the Nogo-NogoReceptor (NgR) pathway enhances axonal sprouting and recovery after focal brain infarction, specifically evaluating motor skill recovery and corticofugal axonal plasticity in rats receiving intracerebroventricular NgR function-blocking fragment after middle cerebral artery occlusion
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Middle Cerebral Artery Occlusion Surgery
Perform focal brain infarction via middle cerebral artery occlusion in rats
Note: This is the initial stroke induction procedure
View evidence from paper
“In rats with middle cerebral artery occlusion”
Intracerebroventricular NgR Fragment Administration
Administer function-blocking NgR fragment via intracerebroventricular injection
Note: Therapy is initiated at 1 week post-stroke, representing delayed pharmacological intervention
View evidence from paper
“Behavioral improvement occurs when therapy is initiated 1 week after arterial occlusion”
Motor Skill Recovery Assessment
Evaluate recovery of complex motor function following NgR blockade treatment
Note: Behavioral testing to measure motor skill recovery
View evidence from paper
“recovery of motor skills and corticofugal axonal plasticity are promoted by intracerebroventricular administration”
Corticofugal Axonal Plasticity Analysis
Examine axonal sprouting and plasticity, specifically measuring axons emanating from undamaged cortex crossing midline to innervate contralateral red nucleus and ipsilateral cervical spinal cord
Note: Anatomical assessment of axonal connections and plasticity
View evidence from paper
“greater numbers of axons emanating from the undamaged cortex cross the midline to innervate the contralateral red nucleus and the ipsilateral cervical spinal cord”