Source Paper
Sleep Deprivation Causes Behavioral, Synaptic, and Membrane Excitability Alterations in Hippocampal Neurons
Carmel M. McDermott, Gerald J. LaHoste, Chu Chen, Alberto Musto, Nicolas G. Bazan et al.
Journal of Neuroscience • 2003
Morris Water Maze
Objective: To assess the impact of sleep deprivation on hippocampus-dependent spatial learning and memory performance using the Morris Water Maze task
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Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Sleep Deprivation Protocol
Rats were exposed to 72 hours of primarily rapid eye movement sleep deprivation
Note: Multiple sleep deprivation methods were used to differentiate effects of sleep deprivation from nonspecific stress
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD impaired their subsequent performance on a hippocampus-dependent spatial learning task”
Morris Water Maze Testing
Following sleep deprivation, rats were tested on the hippocampus-dependent spatial learning task
Note: Performance was measured to assess cognitive impairment from sleep deprivation
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD impaired their subsequent performance on a hippocampus-dependent spatial learning task”
Control Task Testing
Rats were also tested on an amygdala-dependent learning task as a control
Note: Sleep deprivation had no effect on amygdala-dependent learning, indicating hippocampus-specific impairment
View evidence from paper
“had no effect on an amygdala-dependent learning task”