Source Paper
Sleep Deprivation Causes Behavioral, Synaptic, and Membrane Excitability Alterations in Hippocampal Neurons
Carmel M. McDermott, Gerald J. LaHoste, Chu Chen, Alberto Musto, Nicolas G. Bazan et al.
Journal of Neuroscience • 2003
Morris Water Maze
Objective: To assess the impact of sleep deprivation on hippocampus-dependent spatial learning and memory performance using the Morris Water Maze task
This is a Morris Water Maze protocol using rat as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~144 hours hands-on
Core workflow
Sleep Deprivation Protocol • Morris Water Maze Testing • Control Task Testing
Primary readouts
- Performance on hippocampus-dependent spatial learning task
- Performance on amygdala-dependent learning task
- Neuronal excitability in CA1 pyramidal neurons
- Neuronal excitability in dentate gyrus granule cells
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Sleep Deprivation Protocol
Rats were exposed to 72 hours of primarily rapid eye movement sleep deprivation
Note: Multiple sleep deprivation methods were used to differentiate effects of sleep deprivation from nonspecific stress
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD impaired their subsequent performance on a hippocampus-dependent spatial learning task”
Morris Water Maze Testing
Following sleep deprivation, rats were tested on the hippocampus-dependent spatial learning task
Note: Performance was measured to assess cognitive impairment from sleep deprivation
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD impaired their subsequent performance on a hippocampus-dependent spatial learning task”
Control Task Testing
Rats were also tested on an amygdala-dependent learning task as a control
Note: Sleep deprivation had no effect on amygdala-dependent learning, indicating hippocampus-specific impairment
View evidence from paper
“had no effect on an amygdala-dependent learning task”