Morris Water Maze - Cued Response Task
Objective: To determine whether Arc immediate-early gene expression in the hippocampus correlates with learning of a hippocampal-independent cued response water task, compared to spatial learning tasks
This is a Morris Water Maze - Cued Response Task protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rat • not specified • unknown • not specified • not specified
Study window
Estimated timing pending
Core workflow
Cued Response Task Training • RNA Extraction and Analysis • In Situ Hybridization
Primary readouts
- Arc RNA expression levels in hippocampus
- c-fos RNA expression levels
- zif268 RNA expression levels
- Correlation between Arc RNA levels and cued response task learning
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Cued Response Task Training
Rats were trained in a hippocampal-independent cued response water task
Note: This is a nonspatial water task that does not depend on hippocampal function
View evidence from paper
“Rats were trained in a hippocampal-independent cued response water task”
RNA Extraction and Analysis
IEG RNA levels were determined from trained rats using RNase protection assays
Note: Measured Arc, c-fos, and zif268 RNA levels
View evidence from paper
“IEG RNA levels from rats trained in spatial and nonspatial water tasks were determined using RNase protection assays”
In Situ Hybridization
In situ hybridization was performed to analyze IEG expression patterns
Note: Analyzed expression in hippocampus, entorhinal cortex, and primary visual cortex
View evidence from paper
“IEG RNA levels from rats trained in spatial and nonspatial water tasks were determined using RNase protection assays and in situ hybridization”
Correlation Analysis
Arc RNA levels in hippocampus were compared between spatial and cued response tasks
Note: Arc RNA levels were correlated with learning of hippocampal-dependent spatial task but not hippocampal-independent cued response task
View evidence from paper
“Hippocampal Arc RNA levels were correlated with learning of the hippocampal-dependent spatial, but not hippocampal-independent cued response, water task”