Source Paper
High‐mobility Group Protein 1/ Receptor for Advanced Glycation End Products/ Nuclear Factor‐κB Signalling Pathway Contributes to the Pathogenic Process of Striatal Neuron Impairment in the Rat Model of Parkinson's Disease
Zhu Y, Du Z, Sun L, Huang Z, Jia L et al.
Brain Behav • 2026
Morris Water Maze Task
Objective: To assess spatial learning and memory performance in rats using the Morris water maze
This is a Morris Water Maze Task protocol using rat as the model organism. The procedure involves 9 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Brain Behav.
Model and subjects
rat • Sprague-Dawley (SD) • not specified • 8 weeks • 50
Study window
~5 week study window | ~4 minutes hands-on
Core workflow
Timing of experiment • Training protocol • Platform setup
Primary readouts
- Latency to reach target platform
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Timing of experiment
Conduct Morris water maze experiment 5 weeks after 6-OHDA injection
View evidence from paper
“This experiment was conducted 5 weeks after the 6‐OHDA injection”
Training protocol
Train rats in Morris water maze twice daily for five consecutive days
View evidence from paper
“Initially, the rats underwent training in the Morris water maze twice daily for five consecutive days”
Platform setup
Position a 10 cm diameter target platform in a fixed location within the maze
View evidence from paper
“The target platform, which had a diameter of 10 cm, was positioned in a fixed location”
Starting positions
Release animals from four predetermined starting places (east, south, west, and north)
View evidence from paper
“Each experiment involved releasing the animals from four predetermined starting places (east, south, west, and north)”
Swimming trial duration
Allow rats to swim for up to 2 minutes or until they reach the target platform
View evidence from paper
“letting them swim for up to 2 min or until they reached the target platform”
Successful platform finding
If rat finds platform, allow it to remain on platform for 30 seconds to help remember the position
View evidence from paper
“If a rat successfully found and climbed onto the platform, it remained on it for 30 s to help it remember the position”
Failed platform finding
If rat cannot find platform within 2 minutes, manually transfer it to platform and give 30 seconds to stay
View evidence from paper
“If a rat couldn't find the platform within 2 min, it was manually transferred there and given 30 s to stay”
Probe test
Administer probe test on the final day of training
View evidence from paper
“On the final day of training, a probe test was administered”
Data recording
Record latency using TopScan behavioral analysis system during the test
View evidence from paper
“During the test, the TopScan behavioral analysis system (Clever Sys. Inc., USA) was used to record the latency”