Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Morris Water Maze Test
Objective: Spatial memory behavioral test
This is a Morris Water Maze Test protocol using Mice as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 3 materials. Extracted from a 2025 paper published in Cell Death Dis.
Model and subjects
Mice • C57BL/6 J and SAMP8 mice • Male and female • 3 and 20-month-old C57BL/6 J mice, and 2 and 10-month-old SAMP8 mice • 40
Study window
~1.4 week study window
Core workflow
Training setup • Trial parameters • Training schedule
Primary readouts
- Latency time to reach platform (seconds)
- Time spent in each quadrant (seconds)
- Spatial acuity score (calculated from quadrant time and platform area time)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Training setup
Use submerged platform 1 cm below water surface, invisible to animal
View evidence from paper
“submerged 9 cm platform (1 cm below the surface, invisible to the animal)”
Trial parameters
Set maximum trial duration to 60 seconds with 2 seconds on platform at end
View evidence from paper
“maximum trial duration of 60 s and 2 s on the platform”
Training schedule
Train animals with three trials per day for 10 days, excluding weekends
View evidence from paper
“three trials per day for 10 days except for weekends”
Platform location training
Train each animal to locate the hidden platform
View evidence from paper
“Each animal was trained to locate the platform”
Probe test
Conduct probe test on day 11 with platform removed
View evidence from paper
“probe test was carried out on day 11, when the platform was removed”
Animal handling
Remove mouse gently from maze and return to cage after testing
View evidence from paper
“mouse was gently removed from the maze and returned to its cage”