Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Morris Water Maze Test
behavioralMiceC57BL/6 J and SAMP8 mice
Objective: Spatial memory behavioral test
Protocol Steps
1
Training setup
Use submerged platform 1 cm below water surface, invisible to animal
View evidence from paper
“submerged 9 cm platform (1 cm below the surface, invisible to the animal)”
2
Trial parameters
Set maximum trial duration to 60 seconds with 2 seconds on platform at end
View evidence from paper
“maximum trial duration of 60 s and 2 s on the platform”
3
Training schedule
Train animals with three trials per day for 10 days, excluding weekends
View evidence from paper
“three trials per day for 10 days except for weekends”
4
Platform location training
Train each animal to locate the hidden platform
View evidence from paper
“Each animal was trained to locate the platform”
5
Probe test
Conduct probe test on day 11 with platform removed
View evidence from paper
“probe test was carried out on day 11, when the platform was removed”
6
Animal handling
Remove mouse gently from maze and return to cage after testing
View evidence from paper
“mouse was gently removed from the maze and returned to its cage”