Source Paper
Chronic Organic Magnesium Supplementation Enhances Tissue-Specific Bioavailability and Functional Capacity in Rats: A Focus on Brain, Muscle, and Vascular Health
Koc B, Hosgorler F, Kandis S, Acikgoz B, Kizildag S et al.
Biol Trace Elem Res • 2025
Morris Water Maze Test
Objective: To evaluate spatial learning and memory performance
Protocol Steps
Setup tank and quadrants
Divide the Morris water tank into four equal quadrants (SE, SW, NE, NW) using intersecting lines forming a cross shape
View evidence from paper
“Morris water tank was divided into four equal quadrants, southeast (SE), southwest (SW), northeast (NE), and northwest (NW)”
Position platform
Place the platform in the southwest quadrant, 1-2 cm below water surface, keeping position unchanged during learning trials
View evidence from paper
“platform was placed in the southwest quadrant of the tank, 1–2 cm below the water surface”
Conduct learning trials
Place subjects into water from different points each day, facing tank walls. Allow 60 seconds to find platform
View evidence from paper
“subjects were placed into the water from a different point each day, facing the tank's walls”
Handle unsuccessful trials
If subject cannot find platform within 60 seconds, place it on platform and allow 30 seconds observation
View evidence from paper
“If the subject could not find the hidden platform within 60 s, it was placed on the hidden platform”
Continue training
Continue learning trials using this method for four consecutive days
View evidence from paper
“Learning trials with this method continued for four days”
Perform probe trial
On fifth day, remove platform and allow subject to swim freely for 60 seconds
View evidence from paper
“On the fifth day, a probe trial was performed on each animal”