Source Paper
Synergistic Effects of Transplanted Adult Neural Stem/Progenitor Cells, Chondroitinase, and Growth Factors Promote Functional Repair and Plasticity of the Chronically Injured Spinal Cord
Soheila Karimi-Abdolrezaee, Eftekhar Eftekharpour, Jian Wang, Desiree Schut, Michael G. Fehlings
Journal of Neuroscience • 2010
Neurobehavioral Assessment
Objective: Assessment of functional recovery and neurobehavioral outcomes following combined ChABC, growth factor, and NPC treatment in chronic spinal cord injury
This is a Neurobehavioral Assessment protocol using rat as the model organism. The procedure involves 3 procedural steps, 5 materials. Extracted from a 2010 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
ChABC infusion • NPC transplantation and growth factor infusion • Neurobehavioral assessment
Primary readouts
- Functional recovery following chronic spinal cord injury
- NPC survival and integration in host spinal cord
- NPC migration within spinal cord
- NPC differentiation into oligodendrocytes
Key equipment and reagents
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Protocol Steps
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ChABC infusion
Sustained infusion of chondroitinase ABC to target chondroitin sulfate proteoglycans in the glial scar around the chronic spinal cord injury site
Note: Initial treatment targeting CSPGs
View evidence from paper
“We targeted CSPGs in the chronically injured spinal cord by sustained infusion of chondroitinase ABC (ChABC)”
NPC transplantation and growth factor infusion
One week after ChABC infusion, rats received transplants of neural stem/progenitor cells and transient infusion of growth factors (EGF, bFGF, and PDGF-AA)
Note: Combined treatment strategy
View evidence from paper
“One week later, the same rats were treated with transplants of NPCs and transient infusion of growth factors, EGF, bFGF, and PDGF-AA”
Neurobehavioral assessment
Assessment of functional recovery and neurobehavioral outcomes following combined treatment
Note: Measures functional repair and plasticity
View evidence from paper
“These neuroanatomical changes were also associated with significantly improved neurobehavioral recovery after chronic SCI”