Neurological Function Assessment
Objective: Evaluation of neurological performance and recovery following focal cerebral ischemia induced by middle cerebral artery occlusion, with assessment of VEGF effects on neuroprotection, neurogenesis, and angiogenesis
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
not specified • not mentioned • not mentioned • not mentioned
Materials3
Software1
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Protocol Steps
Focal Cerebral Ischemia Induction
Produce focal cerebral ischemia by middle cerebral artery occlusion in adult rat brain
Note: MCAO procedure performed on adult rats
View evidence from paper
“We produced focal cerebral ischemia by middle cerebral artery occlusion for 90 minutes in the adult rat brain”
Reperfusion and VEGF Administration
Allow reperfusion following ischemia and administer VEGF intracerebroventricularly
Note: VEGF administered on days 1, 2, and 3 following reperfusion
View evidence from paper
“intracerebroventricular administration of VEGF on days 1–3 of reperfusion”
Infarct Size Measurement
Measure infarct size in brain tissue following ischemia
Note: Performed with and without VEGF treatment
View evidence from paper
“measured infarct size, neurological function”
Neurological Function Assessment
Evaluate neurological performance and recovery
Note: Assessed with and without VEGF administration
View evidence from paper
“measured infarct size, neurological function”
BrdU Labeling of Neuroproliferative Zones
Label neuroproliferative zones with BrdU to identify newborn neurons
Note: Assesses neurogenesis in dentate gyrus and subventricular zone
View evidence from paper
“BrdU labeling of neuroproliferative zones”
Vascular Profile Assessment
Visualize and quantify vascular profiles using vWF-immunoreactive markers
Note: Measures angiogenesis in striatal ischemic penumbra and dentate gyrus
View evidence from paper
“vWF-immunoreactive vascular profiles”