Neurological Severity Score
Objective: Evaluation of neurological deficits and functional recovery after traumatic brain injury using neurological severity score assessment
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Protocol Steps
Traumatic brain injury induction
Wistar rats were subjected to TBI
Note: Baseline procedure before treatment
View evidence from paper
“Wistar rats were subjected to TBI”
Treatment administration
Tail vein injection of either 100 μg protein of exosomes derived from MSCs or equal volume of vehicle phosphate-buffered saline
Note: Two treatment groups: exosome-treated (n=8) and vehicle control (n=8)
View evidence from paper
“tail vein injection of 100 μg protein of exosomes derived from MSCs or an equal volume of vehicle phosphate-buffered saline (n = 8/group) 24 hours later”
Behavioral testing - Morris water maze
Modified Morris water maze test performed to evaluate cognitive and sensorimotor functional recovery
Note: Timing of testing relative to TBI not specified
View evidence from paper
“To evaluate cognitive and sensorimotor functional recovery, the modified Morris water maze, neurological severity score and footfault tests were performed”
Behavioral testing - Neurological severity score
Neurological severity score assessment performed to evaluate neurological deficits
Note: Specific scoring criteria not detailed in methods
View evidence from paper
“the modified Morris water maze, neurological severity score and footfault tests were performed”
Behavioral testing - Footfault test
Footfault test performed to evaluate sensorimotor functional recovery
Note: Test apparatus and specific procedures not detailed
View evidence from paper
“the modified Morris water maze, neurological severity score and footfault tests were performed”
Animal sacrifice
Animals were sacrificed for tissue collection and analysis
Note: Timing is 35 days post-TBI
View evidence from paper
“Animals were sacrificed at 35 days after TBI”
Histopathological analysis
Histopathological analyses performed for measurements of lesion volume
Note: Performed on tissue collected at sacrifice
View evidence from paper
“Histopathological and immunohistochemical analyses were performed for measurements of lesion volume”
Immunohistochemical analysis
Immunohistochemical analyses performed to measure neurovascular remodeling (angiogenesis and neurogenesis) and neuroinflammation
Note: Performed on tissue collected at sacrifice
View evidence from paper
“Histopathological and immunohistochemical analyses were performed for measurements of lesion volume, neurovascular remodeling (angiogenesis and neurogenesis), and neuroinflammation”