Source Paper
Activated Microglia Contribute to the Maintenance of Chronic Pain after Spinal Cord Injury
Bryan C. Hains, Stephen G. Waxman
Journal of Neuroscience • 2006
Nociceptive Threshold Testing
Objective: Assessment of behavioral nociceptive thresholds to mechanical and thermal stimuli in rats with spinal cord injury
This is a Nociceptive Threshold Testing protocol using rat as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2006 paper published in Journal of Neuroscience.
Model and subjects
rat • Sprague Dawley • male • adult
Study window
Estimated timing pending
Core workflow
Spinal cord injury induction • Nociceptive threshold assessment - mechanical stimuli • Nociceptive threshold assessment - thermal stimuli
Primary readouts
- Behavioral nociceptive thresholds to mechanical stimuli
- Behavioral nociceptive thresholds to thermal stimuli
- Changes in nociceptive thresholds following treatment intervention
Key equipment and reagents
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Protocol Steps
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Spinal cord injury induction
Adult male Sprague Dawley rats underwent T9 spinal cord contusion injury
Note: Baseline for subsequent nociceptive testing
View evidence from paper
“adult male Sprague Dawley rats underwent T9 spinal cord contusion injury”
Nociceptive threshold assessment - mechanical stimuli
Behavioral nociceptive thresholds to mechanical stimuli were measured four weeks after spinal cord injury
Note: Testing performed when lumbar dorsal horn multireceptive neurons became hyperresponsive
View evidence from paper
“Four weeks after injury, when lumbar dorsal horn multireceptive neurons became hyperresponsive and when behavioral nociceptive thresholds were decreased to both mechanical and thermal stimuli”
Nociceptive threshold assessment - thermal stimuli
Behavioral nociceptive thresholds to thermal stimuli were measured four weeks after spinal cord injury
Note: Testing performed when lumbar dorsal horn multireceptive neurons became hyperresponsive
View evidence from paper
“Four weeks after injury, when lumbar dorsal horn multireceptive neurons became hyperresponsive and when behavioral nociceptive thresholds were decreased to both mechanical and thermal stimuli”