Source Paper
Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. Contribution to alterations of vasomotor tone.
S Rajagopalan, S Kurz, T Münzel, M Tarpey, B A Freeman et al.
Journal of Clinical Investigation • 1996
Norepinephrine Infusion
Objective: To induce hypertension via norepinephrine infusion as a control comparison for angiotensin II-induced hypertension effects on vascular superoxide production
This is a Norepinephrine Infusion protocol using rat as the model organism. The procedure involves 2 procedural steps, 2 equipment items, 1 materials. Extracted from a 1996 paper published in Journal of Clinical Investigation.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Norepinephrine infusion initiation • Blood pressure monitoring
Primary readouts
- Systolic blood pressure increase
- Vascular superoxide production (assessed by lucigenin chemiluminescence)
- Vascular relaxation responses to acetylcholine, calcium ionophore A23187, and nitroglycerin
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Norepinephrine infusion initiation
Administer norepinephrine via systemic infusion at specified dose
Note: Dose of 2.75 mg/kg per day produces hypertension comparable to angiotensin II
View evidence from paper
“NE infusion (2.75 mg/kg per d) produced a similar degree of hypertension”
Blood pressure monitoring
Measure systolic blood pressure to confirm hypertension induction
Note: Used as comparison control for angiotensin II effects
View evidence from paper
“Infusion of angiotensin II (0.7 mg/kg per d) increased systolic blood pressure”