Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Novel Object Recognition
Objective: To assess recognition memory by measuring preference for novel versus familiar objects
This is a Novel Object Recognition protocol using Mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 4 materials. Extracted from a 2025 paper published in Cell Death Dis.
Model and subjects
Mice • C57BL/6 J and SAMP8 • Male and female • 3 and 20-month-old C57BL/6 J mice, and 2 and 10-month-old SAMP8 mice • 40
Study window
~2 hour study window
Core workflow
Habituation • Familiarization phase • Inter-trial interval
Primary readouts
- Recognition index calculated as time spent exploring new object divided by time spent exploring both objects
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Habituation
Allow animals to habituate in the empty testing box
View evidence from paper
“The animals were habituated in the box for 5 minutes twice on the first day without any object”
Familiarization phase
Place animal in box with two identical objects for exploration
View evidence from paper
“each animal was placed in the box containing two identical objects (old objects) for 10 min”
Inter-trial interval
Wait 2 hours between familiarization and test phases
View evidence from paper
“After 2 h, the animal was exposed to one of the old objects and a new object”
Test phase
Expose animal to one familiar object and one novel object of different shape and color
View evidence from paper
“the animal was exposed to one of the old objects and a new object of different shape and color”
Cleaning between subjects
Clean box and objects with ethanol between each animal
View evidence from paper
“The box and objects were cleaned (50% ethanol) between each animal testing”