Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Novel Object Recognition
behavioralMiceC57BL/6 J and SAMP8
Objective: To assess recognition memory by measuring preference for novel versus familiar objects
Materials & Equipment Checklist
5 items1 from ConductScience
Protocol Steps
1
Habituation
Allow animals to habituate in the empty testing box
View evidence from paper
“The animals were habituated in the box for 5 minutes twice on the first day without any object”
2
Familiarization phase
Place animal in box with two identical objects for exploration
View evidence from paper
“each animal was placed in the box containing two identical objects (old objects) for 10 min”
3
Inter-trial interval
Wait 2 hours between familiarization and test phases
View evidence from paper
“After 2 h, the animal was exposed to one of the old objects and a new object”
4
Test phase
Expose animal to one familiar object and one novel object of different shape and color
View evidence from paper
“the animal was exposed to one of the old objects and a new object of different shape and color”
5
Cleaning between subjects
Clean box and objects with ethanol between each animal
View evidence from paper
“The box and objects were cleaned (50% ethanol) between each animal testing”