Novel-Object Recognition Test
Objective: Assess recognition memory and cognitive function by measuring animals' ability to discriminate between familiar and novel objects
This is a Novel-Object Recognition Test protocol using rat as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2010 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified in provided text • unknown • middle-aged • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Animal exposure to novel objects • CRF1 receptor antagonist administration • Memory performance assessment
Primary readouts
- Recognition memory performance
- Ability to discriminate between novel and familiar objects
- Cognitive function in early-life stressed animals
- Effects of CRF1 receptor antagonist on memory
Key equipment and reagents
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Protocol Steps
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Animal exposure to novel objects
Animals are exposed to novel objects in the testing apparatus to assess their recognition memory and ability to discriminate between familiar and novel stimuli
Note: Testing was conducted on middle-aged early-life stressed (CES) rats and control animals
View evidence from paper
“improved memory performance of CES rats in novel-object recognition tests”
CRF1 receptor antagonist administration
Central or peripheral administration of a CRF1 blocker following the stress period to test effects on memory performance
Note: Administration was post-hoc, following the initial stress period
View evidence from paper
“Central or peripheral administration of a CRF1 blocker following the stress period improved memory performance of CES rats”
Memory performance assessment
Evaluation of recognition memory performance in novel-object recognition tests following CRF1 antagonist treatment
Note: Performance was compared between treated and untreated animals
View evidence from paper
“Central or peripheral administration of a CRF1 blocker following the stress period improved memory performance of CES rats in novel-object recognition tests”