Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Novel Object Recognition Test
Objective: To assess recognition memory by measuring the time animals spend exploring a novel object versus a familiar object
This is a Novel Object Recognition Test protocol using Mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 4 materials. Extracted from a 2025 paper published in Cell Death Dis.
Model and subjects
Mice • C57BL/6J and SAMP8 • Male and female • 3 and 20-month-old C57BL/6J mice, and 2 and 10-month-old SAMP8 mice • 40
Study window
~2 hour study window | ~15 minutes hands-on
Core workflow
Habituation • Familiarization phase • Recognition test
Primary readouts
- Recognition index calculated as the time spent by the mouse exploring the new object divided by the time spent exploring both objects
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Habituation
Animals were habituated in the box for 5 minutes twice on the first day without any object
View evidence from paper
“The animals were habituated in the box for 5 minutes twice on the first day without any object”
Familiarization phase
Each animal was placed in the box containing two identical objects for 10 minutes
View evidence from paper
“each animal was placed in the box containing two identical objects (old objects) for 10 min”
Recognition test
After 2 hours, the animal was exposed to one old object and a new object of different shape and color
View evidence from paper
“After 2 h, the animal was exposed to one of the old objects and a new object of different shape and color”
Cleaning
The box and objects were cleaned with 50% ethanol between each animal testing
View evidence from paper
“The box and objects were cleaned (50% ethanol) between each animal testing”