Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Novel Object Recognition Test
behavioralMiceC57BL/6J and SAMP8
Objective: To assess recognition memory by measuring the time animals spend exploring a novel object versus a familiar object
Materials & Equipment Checklist
5 items1 from ConductScience
Protocol Steps
1
Habituation
Animals were habituated in the box for 5 minutes twice on the first day without any object
View evidence from paper
“The animals were habituated in the box for 5 minutes twice on the first day without any object”
2
Familiarization phase
Each animal was placed in the box containing two identical objects for 10 minutes
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“each animal was placed in the box containing two identical objects (old objects) for 10 min”
3
Recognition test
After 2 hours, the animal was exposed to one old object and a new object of different shape and color
View evidence from paper
“After 2 h, the animal was exposed to one of the old objects and a new object of different shape and color”
4
Cleaning
The box and objects were cleaned with 50% ethanol between each animal testing
View evidence from paper
“The box and objects were cleaned (50% ethanol) between each animal testing”