Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats

Sebastian Jessberger, Robert E. Clark, Nicola J. Broadbent, Gregory D. Clemenson, Antonella Consiglio et al.

Learning & Memory • 2009

Object Recognition Task

behavioralratadult male rats

Objective: Measure object recognition memory in adult rats with reduced neurogenesis in the dentate gyrus

Materials & Equipment Checklist

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Lentiviral vector expressing dominant-negative WNT (dnWNT)

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Administer lentiviral vector expressing dominant-negative WNT to block neurogenesis in the dentate gyrus of adult male rats

Note: This creates the experimental condition of reduced neurogenesis for testing

View evidence from paper

“lentiviral approach to specifically block neurogenesis in the dentate gyrus of adult male rats by inhibiting WNT signaling”

Conduct hippocampus-dependent object recognition task to assess memory in animals with reduced neurogenesis compared to control rats

Note: Animals with strongly reduced levels of neurogenesis were impaired in this task

View evidence from paper

“animals with strongly reduced levels of neurogenesis were impaired in a hippocampus-dependent object recognition task”