Source Paper
Mateus M Bergamaschi, Regina Helena Costa Queiroz, Marcos Hortes Nisihara Chagas, Danielle Chaves Gomes de Oliveira, Bruno Spinosa De Martinis et al.
Neuropsychopharmacology • 2011
Objective: Measurement of blood pressure, heart rate, and skin conductance at six time points during a simulated public speaking test to assess physiological anxiety responses
This is a Physiological Monitoring protocol using human as the model organism. The procedure involves 6 procedural steps, 3 equipment items, 2 materials. Extracted from a 2011 paper published in Neuropsychopharmacology.
Model and subjects
human • N/A • Not specified • Not specified • Not specified • 48
Study window
~1.5 hours hands-on
Core workflow
Pre-treatment administration • Baseline physiological measurement (Time point 1) • Simulated public speaking test (SPST)
Primary readouts
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Administer CBD (600 mg) or placebo to SAD patients in double-blind randomized design
Note: Double-blind procedure; healthy controls receive no medication
“24 never-treated patients with SAD were allocated to receive either CBD (600 mg; n=12) or placebo (placebo; n=12) in a double-blind randomized design 1 h and a half before the test”
Measure blood pressure, heart rate, and skin conductance at baseline before simulated public speaking test begins
Note: First of six measurement time points
“physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points during the SPST”
Conduct simulated public speaking test with participants
Note: Test conducted with all three groups: CBD-treated SAD patients, placebo-treated SAD patients, and healthy controls
“Each volunteer participated in only one experimental session in a double-blind procedure”
Measure blood pressure, heart rate, and skin conductance at five additional time points during the simulated public speaking test
Note: Total of six measurement time points throughout the test
“physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points during the SPST”
Administer Visual Analogue Mood Scale (VAMS) to assess subjective anxiety, cognitive impairment, discomfort, and alert levels
Note: Administered at the same six time points as physiological measures
“Subjective ratings on the Visual Analogue Mood Scale (VAMS) and Negative Self-Statement scale (SSPS-N) and physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points”
Administer Negative Self-Statement scale (SSPS-N) to assess cognitive anxiety and negative self-talk during the test
Note: Administered at the same six time points as physiological measures
“Subjective ratings on the Visual Analogue Mood Scale (VAMS) and Negative Self-Statement scale (SSPS-N) and physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Measurement of blood pressure, heart rate, and skin conductance at six time points during a simulated public speaking test to assess physiological anxiety responses
Objective
Measurement of blood pressure, heart rate, and skin conductance at six time points during a simulated public speaking test to assess physiological anxiety responses
Subjects
From paperhuman • N/A • Not specified • Not specified • Not specified
Sample count
From paper48
Cohort notes
From paper24 treatment-naive SAD patients (12 CBD, 12 placebo) and 12 healthy controls; double-blind randomized design
Pre-treatment administration (1 hour and 30 minutes before test)
Baseline physiological measurement (Time point 1) (Not specified)
Simulated public speaking test (SPST) (Not specified)
Physiological measurements during SPST (Time points 2-6) (Not specified)
Blood pressure (systolic and diastolic)
From paperResults were submitted to a repeated-measures analysis of variance
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Heart rate
From paperResults were submitted to a repeated-measures analysis of variance
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Skin conductance
From paperResults were submitted to a repeated-measures analysis of variance
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Visual Analogue Mood Scale (VAMS) scores for anxiety, cognitive impairment, discomfort, and alert
From paperResults were submitted to a repeated-measures analysis of variance
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Blood pressure (systolic and diastolic)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Heart rate
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Skin conductance
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Visual Analogue Mood Scale (VAMS) scores for anxiety, cognitive impairment, discomfort, and alert
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
Results were submitted to a repeated-measures analysis of variance
Scoring or quantification
Quantify the primary readouts for this experiment: Blood pressure (systolic and diastolic); Heart rate; Skin conductance; Visual Analogue Mood Scale (VAMS) scores for anxiety, cognitive impairment, discomfort, and alert.
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Blood pressure (systolic and diastolic), Heart rate, Skin conductance, Visual Analogue Mood Scale (VAMS) scores for anxiety, cognitive impairment, discomfort, and alert.
Source links and direct wording from the methods section for validation and deeper review.
Citation
Mateus M Bergamaschi et al. (2011). Cannabidiol Reduces the Anxiety Induced by Simulated Public Speaking in Treatment-Naïve Social Phobia Patients. Neuropsychopharmacology
Pre-treatment administration • Protocol step
“24 never-treated patients with SAD were allocated to receive either CBD (600 mg; n=12) or placebo (placebo; n=12) in a double-blind randomized design 1 h and a half before the test”
Baseline physiological measurement (Time point 1) • Protocol step
“physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points during the SPST”
Simulated public speaking test (SPST) • Protocol step
“Each volunteer participated in only one experimental session in a double-blind procedure”
Subjective mood assessment • Protocol step
“Subjective ratings on the Visual Analogue Mood Scale (VAMS) and Negative Self-Statement scale (SSPS-N) and physiological measures (blood pressure, heart rate, and skin conductance) were measured at six different time points”
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Current status surfaces were computed from experiment data updated Feb 28, 2026.
Source access
Jump back into the original paper or the methods evidence section when you need exact wording, exclusions, or method-specific caveats.
This protocol has structured steps plus evidence quotes, and is ready for canonical sync.
Steps
6
Evidence Quotes
4
Protocol Items
5
Linked Products
0
Canonical Sync
Pending
What this means
The completeness score reflects how much structured protocol data is present: steps, methods evidence, listed materials, linked products, and paper provenance.
Computed from the current experiment record updated Feb 28, 2026.
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Steps
6
Evidence
4
Specific Products
0/0
Canonical Sync
Pending
What this score means
The verification score reflects evidence coverage, subject detail, paper provenance, step depth, and whether linked products resolve to specific item pages instead of generic searches.
Computed from the current experiment record updated Feb 28, 2026.
A page can have structured steps and still need review when evidence is thin, product links are generic, or canonical protocol coverage is still pending.
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