REM Sleep Deprivation with Polygraphic Recording
Objective: Test whether experimentally induced P-wave generator activation could eliminate the learning impairment produced by post-training REM sleep deprivation
This is a REM Sleep Deprivation with Polygraphic Recording protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2004 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~6 hours hands-on
Core workflow
Avoidance-learning task training • Microinjection administration • Polygraphic recording and REM sleep deprivation
Primary readouts
- Performance on two-way active avoidance-learning task
- Learning deficits or normal learning across experimental groups
- Sleep state monitoring during polygraphic recording
Key equipment and reagents
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Protocol Steps
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Avoidance-learning task training
Rats were trained on a two-way active avoidance-learning task
Note: This training occurred prior to microinjection and sleep deprivation
View evidence from paper
“Rats were trained on a two-way active avoidance-learning task. Immediately thereafter, two groups of those rats received a control vehicle”
Microinjection administration
Immediately after training, rats received either saline control (100 nl) or carbachol (50 ng in 100 nl saline) microinjection into the P-wave generator
Note: Two groups received saline control, one group received carbachol
View evidence from paper
“Immediately thereafter, two groups of those rats received a control vehicle (100 nl saline) microinjection and one group received a carbachol (50 ng in 100 nl saline) microinjection into the P-wave generator”
Polygraphic recording and REM sleep deprivation
Carbachol-injected group and one saline-injected control group were selectively deprived of REM sleep during polygraphic recording session
Note: One saline-injected group was allowed to sleep normally as control
View evidence from paper
“The carbachol-injected group and one of the two control saline microinjected groups were selectively deprived of REM sleep during a 6 hr polygraphic recording session”
Avoidance-learning task retesting
All rats were tested on the avoidance-learning task following the sleep deprivation/recording session
Note: Performance was compared across groups to assess learning impairment
View evidence from paper
“All rats were then tested on the avoidance-learning task”