Source Paper
Neurotoxic Lesions of Basolateral, But Not Central, Amygdala Interfere with Pavlovian Second-Order Conditioning and Reinforcer Devaluation Effects
Tammy Hatfield, Jung-Soo Han, Michael Conley, Michela Gallagher, Peter Holland
Journal of Neuroscience • 1996
Second-Order Conditioning
Objective: To examine the effects of selective neurotoxic lesions of basolateral amygdala (ABL) or central nucleus (CN) on acquisition of positive incentive value by a conditioned stimulus through second-order conditioning, measuring the acquired reinforcing power of a light stimulus
This is a Second-Order Conditioning protocol using rat as the model organism. The procedure involves 2 procedural steps. Extracted from a 1996 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
First-Order Conditioning: Light-Food Pairings • Second-Order Conditioning: Tone-Light Pairings
Primary readouts
- Acquisition of second-order conditioning in rats with ABL lesions versus CN lesions versus control rats
- Ability of the light to serve as a reinforcer for tone conditioning
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
First-Order Conditioning: Light-Food Pairings
Rats received light-food pairings intended to endow the light with reinforcing power through classical conditioning
Note: This establishes the light as a conditioned stimulus with acquired reinforcing properties
View evidence from paper
“rats first received light–food pairings intended to endow the light with reinforcing power”
Second-Order Conditioning: Tone-Light Pairings
Tone-light pairings were given in the absence of food to measure the acquired reinforcing power of the light by examining its ability to serve as a reinforcer for second-order conditioning of a tone
Note: The light, now a conditioned stimulus with reinforcing properties from step 1, is used as the reinforcer for the tone
View evidence from paper
“The acquired reinforcing power of the light was then measured by examining its ability to serve as a reinforcer for second-order conditioning of a tone when tone–light pairings were given in the absence of food”