Sleep Recording
Objective: To assess sleep architecture and patterns during chronic episodic hypoxia exposure in rats, and correlate sleep disruption with cognitive and cellular changes
This is a Sleep Recording protocol using rat as the model organism. The procedure involves 4 procedural steps, 3 equipment items. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rat • Sprague Dawley • male • Not specified • Not specified • Not specified
Study window
~2 week study window | ~24.5 hours hands-on
Core workflow
Chronic episodic hypoxia exposure • Sleep recording and architecture assessment • Morris water maze cognitive testing
Primary readouts
- Sleep architecture disruption during initial exposure
- Sleep pattern normalization over time
- Apoptosis levels in CA1 hippocampal region and cortex
- NMDA NR1 glutamate receptor expression
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Chronic episodic hypoxia exposure
Rats were placed in an environmental chamber where oxygen concentrations were cycled between 10% and 21% every 90 seconds or every 30 minutes during 12 hours of daylight
Note: During the remaining 12 hours, EHYP rats breathed room air. Control rats spent 14 days in room air continuously
View evidence from paper
“Exposures consisted of up to 14 d in an environmental chamber in which O2 concentrations were cycled between 10 and 21% every 90 sec or 30 min during 12 hr of daylight”
Sleep recording and architecture assessment
Polysomnographic recordings were conducted to measure sleep architecture and patterns during chronic episodic hypoxia exposure
Note: Sleep patterns were assessed during initial day of exposure and throughout the 14-day exposure period
View evidence from paper
“Sleep recordings, Morris water maze experiments, and immunohistochemistry for NMDA NR1 glutamate receptor, c-fos protein, and apoptosis were conducted in EHYP-exposed Sprague Dawley male rats”
Morris water maze cognitive testing
Rats were trained on a hidden platform task to assess spatial learning and memory acquisition
Note: Escape latencies and swim path lengths were measured as outcome variables
View evidence from paper
“Rats exposed to EHYP displayed significantly longer escape latencies and swim path lengths to escape a hidden platform during 12 training trials given over 2 d”
Recovery period assessment
Cognitive performance was reassessed after 14 days of recovery following the exposure period
Note: Differences in performance between EHYP and control rats persisted although reduced after recovery
View evidence from paper
“Differences in the performances of EHYP and control rats, although reduced, persisted after 14 d of recovery”