Sleep Recording
Objective: To assess sleep architecture and patterns during chronic episodic hypoxia exposure in rats, and correlate sleep disruption with cognitive and cellular changes
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Protocol Steps
Chronic episodic hypoxia exposure
Rats were placed in an environmental chamber where oxygen concentrations were cycled between 10% and 21% every 90 seconds or every 30 minutes during 12 hours of daylight
Note: During the remaining 12 hours, EHYP rats breathed room air. Control rats spent 14 days in room air continuously
View evidence from paper
“Exposures consisted of up to 14 d in an environmental chamber in which O2 concentrations were cycled between 10 and 21% every 90 sec or 30 min during 12 hr of daylight”
Sleep recording and architecture assessment
Polysomnographic recordings were conducted to measure sleep architecture and patterns during chronic episodic hypoxia exposure
Note: Sleep patterns were assessed during initial day of exposure and throughout the 14-day exposure period
View evidence from paper
“Sleep recordings, Morris water maze experiments, and immunohistochemistry for NMDA NR1 glutamate receptor, c-fos protein, and apoptosis were conducted in EHYP-exposed Sprague Dawley male rats”
Morris water maze cognitive testing
Rats were trained on a hidden platform task to assess spatial learning and memory acquisition
Note: Escape latencies and swim path lengths were measured as outcome variables
View evidence from paper
“Rats exposed to EHYP displayed significantly longer escape latencies and swim path lengths to escape a hidden platform during 12 training trials given over 2 d”
Recovery period assessment
Cognitive performance was reassessed after 14 days of recovery following the exposure period
Note: Differences in performance between EHYP and control rats persisted although reduced after recovery
View evidence from paper
“Differences in the performances of EHYP and control rats, although reduced, persisted after 14 d of recovery”