Source Paper
Inhibition of Activity-Dependent Arc Protein Expression in the Rat Hippocampus Impairs the Maintenance of Long-Term Potentiation and the Consolidation of Long-Term Memory
John F. Guzowski, Gregory L. Lyford, Gail D. Stevenson, Frank P. Houston, James L. McGaugh et al.
Journal of Neuroscience • 2000
Spatial Water Task Training
Objective: To investigate the role of Arc protein in hippocampal plasticity by examining effects of Arc protein expression inhibition on long-term potentiation maintenance and spatial water task learning consolidation in rats
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Equipment1
Not specified • Not specified • Not specified • Not specified
Materials1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Intrahippocampal infusion of antisense oligodeoxynucleotides
Administer intrahippocampal infusions of antisense oligodeoxynucleotides to inhibit Arc protein expression
Note: Treatment designed to disrupt Arc protein expression in hippocampus
View evidence from paper
“We used intrahippocampal infusions of antisense oligodeoxynucleotides to inhibit Arc protein expression”
Spatial water task training
Conduct behavioral training on spatial water task to assess long-term memory consolidation and acquisition
Note: Training results in long-term memory formation; task acquisition and short-term memory remain unaffected by Arc inhibition
View evidence from paper
“spatial water task training without affecting task acquisition or short-term memory”
Assessment of long-term potentiation
Examine effects of Arc protein inhibition on both induction and maintenance phases of long-term potentiation
Note: Arc disruption impairs maintenance phase of LTP without affecting induction
View evidence from paper
“disruption of Arc protein expression impairs the maintenance phase of LTP without affecting its induction”