Spinal Cord Contusion Injury Model
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This is a Spinal Cord Contusion Injury Model protocol using rat as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 4 materials. Extracted from a 2014 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~9 week study window
Core workflow
Create contusive spinal cord injury • Neural stem cell transplantation • Treadmill locomotor training
Primary readouts
- Locomotor function recovery
- Survival of grafted neural stem cells
- Cellular stress markers (reactive nitrogen and oxygen species)
- Cerebrospinal fluid IGF-1 concentration
Key equipment and reagents
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Protocol Steps
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Create contusive spinal cord injury
Establish rat contusive spinal cord injury model
Note: Baseline timepoint for subsequent interventions
View evidence from paper
“rat contusive injury model”
Neural stem cell transplantation
Transplant neural stem cells obtained at embryonic day 14 into injured spinal cord
Note: Timing is 1 week post-injury
View evidence from paper
“Combination of TMT with NSC TP at 1 week after injury synergistically improved locomotor function”
Treadmill locomotor training
Implement treadmill training to promote locomotor recovery
Note: Combined with NSC transplantation for synergistic effects
View evidence from paper
“Combination of TMT with NSC TP at 1 week after injury synergistically improved locomotor function”
Assess locomotor function
Measure locomotor recovery at multiple timepoints
Note: Survival of grafted NSCs assessed at 3 and 9 weeks
View evidence from paper
“combining TMT increased the survival of grafted NSCs by >3-fold and >5-fold at 3 and 9 weeks after injury”
Intrathecal antibody infusion
Administer neutralizing IGF-1 antibodies, BDNF antibodies, or NT-3 antibodies via intrathecal infusion
Note: Used to determine which growth factor mediates TMT effects on NSC survival
View evidence from paper
“Intrathecal infusion of neutralizing IGF-1 antibodies, but not antibodies against either BDNF or Neurotrophin-3 (NT-3), abolished the enhanced survival of NSC grafts by TMT”
Measure cerebrospinal fluid IGF-1 concentration
Quantify IGF-1 levels in cerebrospinal fluid
Note: TMT increased CSF IGF-1 concentration
View evidence from paper
“TMT increased the concentration of insulin-like growth factor-1 (IGF-1) in the CSF”
Assess tissue sparing and myelination
Evaluate tissue preservation and myelination in spinal cord
Note: Combination of TP and TMT resulted in greater tissue sparing and myelination
View evidence from paper
“The combination of TP and TMT also resulted in tissue sparing, increased myelination, and restoration of serotonergic fiber innervation”
Assess serotonergic fiber innervation
Measure restoration of serotonergic fiber innervation to lumbar spinal cord
Note: Combination treatment showed greater restoration than either treatment alone
View evidence from paper
“restoration of serotonergic fiber innervation to the lumbar spinal cord to a larger extent than that induced by either TP or TMT alone”