Spinal Cord Injury Model
Objective: Establishment of a spinal cord injury model in rats to evaluate the effects of stem cell-derived extracellular vesicles on recovery
This is a Spinal Cord Injury Model protocol using rat as the model organism. The procedure involves 7 procedural steps, 3 materials. Extracted from a 2023 paper published in Cell Death and Disease.
Model and subjects
rat • Wistar • female • 200-220g • 200
Study window
~4 week study window
Core workflow
Animal housing and environmental conditions • Experimental group assignment • Spinal cord injury model establishment
Primary readouts
- Effects of stem cell-derived extracellular vesicles on spinal cord injury recovery
Key equipment and reagents
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Protocol Steps
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Animal housing and environmental conditions
All female Wistar rats were housed in controlled environments with specific temperature and humidity parameters
Note: Humidity maintained at 60-80%
View evidence from paper
“All female Wistar rats were housed in the same environments (the temperature ranging from 22 to 24°C, the humidity ranging from 60 to 80%)”
Experimental group assignment
Rats were randomly divided into five experimental groups: Sham, SCI, SCI + SCDEs, SCI + SCDEs (shMFG-E8-KO), and SCI + SCDEs (shNC)
Note: Random assignment to groups
View evidence from paper
“Rats were randomly divided into the following groups: Sham, SCI, SCI + SCDEs, SCI + SCDEs (shMFG-E8-KO), and SCI + SCDEs (shNC)”
Spinal cord injury model establishment
Spinal cord injury model was established in rats according to experimental protocol
Note: Specific methodology for SCI induction not detailed in provided text
View evidence from paper
“Half an hour after the spinal cord injury model was established, SCDEs were injected through the tail vein of rats”
Initial SCDE injection (acute phase)
SCDEs were injected through the tail vein 30 minutes after spinal cord injury model establishment
Note: Injection route: tail vein
View evidence from paper
“Half an hour after the spinal cord injury model was established, SCDEs were injected through the tail vein of rats. We injected rats used in the acute phase (3 days) with SCDEs once a day”
SCDE injection (subacute phase)
SCDEs were injected via tail vein during subacute phase of recovery
Note: Injection route: tail vein
View evidence from paper
“Rats in the subacute (2 weeks) and postacute phases (4 weeks) were injected with SCDEs via the tail vein three times per week”
SCDE injection (postacute phase)
SCDEs were injected via tail vein during postacute phase of recovery
Note: Injection route: tail vein
View evidence from paper
“Rats in the subacute (2 weeks) and postacute phases (4 weeks) were injected with SCDEs via the tail vein three times per week”
Control group injection
SCI control group received DPBS vehicle control injection
Note: Injection route: tail vein
View evidence from paper
“the SCI group was injected with the same volume of DPBS”