Spinal Cord Transection and Transplantation
Objective: To evaluate axonal regeneration and functional recovery after complete spinal cord transection in rats using delayed fetal spinal cord transplants and neurotrophic factors
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
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Protocol Steps
Spinal cord transection
Complete midthoracic spinal cord transection performed in adult rats
Note: Transection performed at midthoracic level
View evidence from paper
“Complete midthoracic spinal cord transection in rats with subsequent delayed fetal spinal cord transplant placement”
Acute transplant placement
Fetal spinal cord transplants placed into lesion cavity immediately after transection in acute injury group
Note: One experimental group receiving immediate transplantation
View evidence from paper
“Transplants were placed into the lesion cavity either immediately after transection (acute injury)”
Delayed transplant placement
Fetal spinal cord transplants placed into lesion cavity after 2-4 week delay following transection
Note: Delayed or chronic transplant group showing improved outcomes
View evidence from paper
“or after a 2–4 week delay (delayed or chronic transplants)”
Neurotrophic factor administration
Exogenous neurotrophic factors administered via implanted minipump in treatment groups
Note: Administration timing coordinated with transplant placement (acute or delayed)
View evidence from paper
“either vehicle or neurotrophic factors were administered exogenously via an implanted minipump”
Behavioral assessment
Evaluation of motor function recovery including plantar foot placement and weight-supported stepping
Note: Functional recovery assessed in all treatment groups
View evidence from paper
“animals exhibiting plantar foot placement and weight-supported stepping”