Spinal Cord Transplantation and Locomotor Recovery
Objective: Assessment of hind limb locomotor function recovery following transplantation of human dental pulp stem cells into completely transected rat spinal cord
This is a Spinal Cord Transplantation and Locomotor Recovery protocol using rat as the model organism. The procedure involves 3 procedural steps, 3 materials. Extracted from a 2011 paper published in Journal of Clinical Investigation.
Model and subjects
rat • adult rat • not specified • adult • not specified • not specified
Study window
Estimated timing pending
Core workflow
Spinal cord transection • Cell transplantation • Locomotor function assessment
Primary readouts
- Hind limb locomotor function recovery
- Preservation of neuronal filaments and myelin sheaths
- Axon regeneration
- Cell differentiation into mature oligodendrocytes
Key equipment and reagents
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Protocol Steps
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Spinal cord transection
Complete transection of the adult rat spinal cord performed
Note: Complete transection indicates full severing of the spinal cord
View evidence from paper
“transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord”
Cell transplantation
Transplantation of human dental pulp stem cells into the transected spinal cord
Note: Timing of transplantation relative to injury not specified
View evidence from paper
“transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord resulted in marked recovery of hind limb locomotor functions”
Locomotor function assessment
Assessment of hind limb locomotor function recovery
Note: Specific assessment methods and timeline not detailed in provided text
View evidence from paper
“marked recovery of hind limb locomotor functions”